Team:KULeuven/Tools

From 2008.igem.org

(Difference between revisions)
(→Digestion sheet)
(→Lab Templates)
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'''Usage:'''
'''Usage:'''
-
  <nowiki>{{:Team:KULeuven/Tools/PCR
+
  <nowiki>
-
|tanneal=55
+
{{:Team:KULeuven/Tools/PCR
-
|telong=2'30"
+
|tanneal=55
-
|ncycles=30
+
|telong=2'30"
-
|buffer=5x 10μL
+
|ncycles=30
-
|mgcl=10μM 0μL
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|buffer=5x 10μL
-
|dntp=10μM 1μL
+
|mgcl=10μM 0μL
-
|fwprimername=6 LacZ-Fw
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|dntp=10μM 1μL
-
|fwprimerconc=10μM 2.5μL
+
|fwprimername=6 LacZ-Fw
-
|rvprimername=7 LacZ-Rv
+
|fwprimerconc=10μM 2.5μL
-
|rvprimerconc=10μM 2.5μL
+
|rvprimername=7 LacZ-Rv
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|water=34μL
+
|rvprimerconc=10μM 2.5μL
-
|polymerase=Taq 0.5μL
+
|water=34μL
-
|dna=0.5μL of E. coli DNA extract
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|polymerase=Taq 0.5μL
-
|size=952bp
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|dna=0.5μL of E. coli DNA extract
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|success=YES/NO
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|size=952bp
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|image=pcrproducts_GE.jpg
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|success=YES/NO
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|lane=3+4
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|image=pcrproducts_GE.jpg
-
}}</nowiki>
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|lane=3+4
 +
}}
 +
</nowiki>
'''Result:'''
'''Result:'''
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  <nowiki>
  <nowiki>
  {{:Team:KULeuven/Tools/Digestion
  {{:Team:KULeuven/Tools/Digestion
-
|nr=D12
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|nr=D12
-
|product=Cre ORF
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|product=Cre ORF
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|matrix=pJ61047
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|matrix=pJ61047
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|enz1=Pst1
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|enz1=Pst1
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|enz2=Xba1
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|enz2=Xba1
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|size=1063bp
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|size=1063bp
-
|desc=Cre ORF extracted for further usage
+
|desc=Cre ORF extracted for further usage
-
}}
+
}}
  </nowiki>
  </nowiki>
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'''Usage:'''
'''Usage:'''
 +
<nowiki>
 +
{{:Team:KULeuven/Tools/Ligation
 +
|nr=L21
 +
|insert=D12 Cre ORF
 +
|insert_vol=6µL
 +
|vector=D1 pJ23100
 +
|vector_vol=2µL
 +
|comm=construction of BioBrick: Cre-recombinase under control of strong promotor
 +
|nr_col=30
 +
}}
 +
</nowiki>
 +
 +
'''Result'''
 +
{{:Team:KULeuven/Tools/Ligation
 +
|nr=L21
 +
|insert=D12 Cre ORF
 +
|insert_vol=6µL
 +
|vector=D1 pJ23100
 +
|vector_vol=2µL
 +
|comm=construction of BioBrick: Cre-recombinase under control of strong promotor
 +
|nr_col=30
 +
}}
==CSS==
==CSS==
==== [[User:Avdmeers/SimpleCalendar.css | Calendar CSS file]] ====
==== [[User:Avdmeers/SimpleCalendar.css | Calendar CSS file]] ====

Revision as of 14:05, 3 July 2008

Under Construction...

Contents

Templates

General

Navigation Bar

Usage: 

{{:Team:KULeuven/Tools/Navigation_Bar}}

Lab Templates

PCR sheet

Usage: 

{{:Team:KULeuven/Tools/PCR
|tanneal=55
|telong=2'30"
|ncycles=30
|buffer=5x 10μL
|mgcl=10μM 0μL
|dntp=10μM 1μL
|fwprimername=6 LacZ-Fw
|fwprimerconc=10μM 2.5μL
|rvprimername=7 LacZ-Rv
|rvprimerconc=10μM 2.5μL
|water=34μL
|polymerase=Taq 0.5μL
|dna=0.5μL of E. coli DNA extract
|size=952bp
|success=YES/NO
|image=pcrproducts_GE.jpg
|lane=3+4
}}

Result:

PCR:
PCR Settings
Annealing (°C)
55°
Time Elongation
2'30"
Number of Cycles
30
Buffer (5x) 5x 10μL
MgCl2 10 µM 10μM 0μL
dNTP 10 µM 10μM 1μL
Oligo F 10 µM 6 LacZ-Fw 10μM 2.5μL
Oligo R 10 µM 7 LacZ-Rv 10μM 2.5μL
Water 34μL
Polymerase Taq 0.5μL
DNA 0.5μL of E. coli DNA extract
Expected Size
952bp
Success
YES/NO
Image (click to enlarge)
30px
Lane (0=ladder)
3+4

Digestion sheet

Usage: 

 {{:Team:KULeuven/Tools/Digestion
|nr=D12
|product=Cre ORF
|matrix=pJ61047
|enz1=Pst1
|enz2=Xba1
|size=1063bp
|desc=Cre ORF extracted for further usage
}}

Result:

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D12 Cre ORF pJ61047 XbaI PstI 1063bp Cre ORF extracted for further usage

Ligation sheet

Usage: 

{{:Team:KULeuven/Tools/Ligation
|nr=L21
|insert=D12 Cre ORF
|insert_vol=6µL
|vector=D1 pJ23100
|vector_vol=2µL
|comm=construction of BioBrick: Cre-recombinase under control of strong promotor
|nr_col=30
}}

Result

Ligations
Number Insert Insert Volume (µL) Vector Vector Volume (µL) Comments Number of colonies
L21 D12 Cre ORF 6µL D1 pJ23100 2µL construction of BioBrick: Cre-recombinase under control of strong promotor {{{ncol}}}

CSS

Calendar CSS file

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