Team:KULeuven/Tools

From 2008.igem.org

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Revision as of 18:18, 7 July 2008

Under Construction...

Contents

Templates

General

Navigation Bar

Example Usage: 

{{:Team:KULeuven/Tools/Navigation_Bar}}

The navigation bar used throughout the wiki. The template contains inline CSS and Javascript.
In general, one box in the bar is encoded as:

	<!-- ... SECTION -->
	<li><a href="http://... (menu http address)"
	onmouseover="mopen('m4')" onmouseout="mclosetime()">... (menu name)</a>
		<div id="m4" onmouseover="mcancelclosetime()" onmouseout="mclosetime()">
		<a href="http://... (menuitem http address)</a>
		<a href="http://... (menuitem http address)</a>
		...
		</div>
	</li>
 
The code of the old navigation bar is:

{|style="border-spacing:6px; text-align:center; color:white" width="960px"
|-bgcolor="#003E81" padding="3"
![[Team:KULeuven|Home]]
![[Team:KULeuven/Team|The Team]]
![[Team:KULeuven/Road Map|Road Map]]
![[Team:KULeuven/Project|The Project]]
![[Team:KULeuven/Parts|Parts Submitted to the Registry]]
![[Team:KULeuven/Modeling|Modeling]]
![[Team:KULeuven/Notebook|Notebook]]
|}

New Day

Example Usage: 

{{#calendar: query=preload=:Team:Kuleuven/Tools/New_Day}}

The day template that is preloaded into a notebook day. The result can be seen in the Notebook section, when you
click on an unmodified day, you'll get the edit screen, prefilled with the contents of the New_Day template.

Lab Templates

PCR sheet

Example Usage: 

{{:Team:KULeuven/Tools/PCR
|tanneal=55
|telong=2'30"
|ncycles=30
|buffer=5x 10μL
|mgcl=10μM 0μL
|dntp=10μM 1μL
|fwprimername=6 LacZ-Fw
|fwprimerconc=10μM 2.5μL
|rvprimername=7 LacZ-Rv
|rvprimerconc=10μM 2.5μL
|water=34μL
|polymerase=Taq 0.5μL
|dna=0.5μL of E. coli DNA extract
|size=952bp
|success=YES/NO
|image=pcrproducts_GE.jpg
|lane=3+4
}}

Result:

PCR:
PCR Settings
Annealing (°C)
55°
Time Elongation
2'30"
Number of Cycles
30
Buffer (5x) 5x 10μL
MgCl2 10 µM 10μM 0μL
dNTP 10 µM 10μM 1μL
Oligo F 10 µM 6 LacZ-Fw 10μM 2.5μL
Oligo R 10 µM 7 LacZ-Rv 10μM 2.5μL
Water 34μL
Polymerase Taq 0.5μL
DNA 0.5μL of E. coli DNA extract
Expected Size
952bp
Success
YES/NO
Image (click to enlarge)
30px
Lane (0=ladder)
3+4

Digestion sheet

Example Usage: 

 {{:Team:KULeuven/Tools/Digestion
|nr=D12
|product=Cre ORF
|matrix=pJ61047
|enz1=Pst1
|enz2=Xba1
|size=1063bp
|desc=Cre ORF extracted for further usage
}}

Result:

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description
D12 Cre ORF pJ61047 XbaI PstI 1063bp Cre ORF extracted for further usage

Ligation sheet

Example Usage: 

{{:Team:KULeuven/Tools/Ligation
|nr=L21
|insert=D12 Cre ORF
|insert_vol=6µL
|vector=D1 pJ23100
|vector_vol=2µL
|comm=construction of BioBrick: Cre-recombinase under control of strong promotor
|ncol=30
}}

Result

Ligations
Number Insert Insert Volume (µL) Vector Vector Volume (µL) Comments Number of colonies
L21 D12 Cre ORF 6µL D1 pJ23100 2µL construction of BioBrick: Cre-recombinase under control of strong promotor 30

96-Well Plate

Example Usage: 

{{:Team:KULeuven/Tools/96-well
|title=Induced Response
|A1=LB + 0µM IPTG
|A2=LB + 0µM IPTG
|A3=LB + 5µM IPTG
|A4=LB + 5µM IPTG
|A5=LB + 10µM IPTG
|A6=LB + 10µM IPTG
|A7=etc
|B1=LB 2 + 0µM IPTG
|C1=LB 3 + 0µM IPTG
|D1=LB 4 + 0µM IPTG
|E1=LB 5 + 0µM IPTG
|F1=etc
}}

Result

96-Well Plate: Induced Response
1 2 3 4 5 6 7 8 9 10 11 12
A LB + 0µM IPTGLB + 0µM IPTGLB + 5µM IPTG LB + 5µM IPTGLB + 10µM IPTGLB + 10µM IPTG etc// ///
B LB 2 + 0µM IPTG// /// /// ///
C LB 3 + 0µM IPTG// /// /// ///
D LB 4 + 0µM IPTG// /// /// ///
E LB 5 + 0µM IPTG// /// /// ///
F etc// /// /// ///
G /// /// /// ///
H /// /// /// ///


Collection Templates

Primer Collection

Primer Collection Wiki Page 

To start the primer collection wiki page, simply create a new page and add following lines:

{|width="960" cellspacing="1"
! style="background:#003E81; color: white" colspan="5" | Table of Oligos
|-style="background:#66FFCC" align="center"
| width="40pt" | Number
| width="80pt" | Name
| width="500pt" | Sequence
| width="30pt" | Length
| width="300pt" | Comments
...
|}

Where you see the dots the template codelines will be added to. The dots will be replaced by:

{{:Team:KULeuven/Tools/Oligo ...}}
{{:Team:KULeuven/Tools/Oligo ...}}

Example Usage

Result

CSS

Calendar CSS file

Retrieved from "http://2008.igem.org/Team:KULeuven/Tools"