"
Virginia/9 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: ==Overnight Results== *pSB1A10 screening plasmid grew just fine *Assembly 1 (BP1 + Term) grew more colonies *pSB1AC3 and pSB1AK3 both grew a small number of colonies <br>) |
|||
| Line 4: | Line 4: | ||
*pSB1AC3 and pSB1AK3 both grew a small number of colonies | *pSB1AC3 and pSB1AK3 both grew a small number of colonies | ||
<br> | <br> | ||
| + | ==Goals== | ||
| + | *Transform overnight ligations | ||
| + | **Note: Considering the other ligations didn't work and the restriction digest might be the problem we don't expect much | ||
| + | *Make small (10ml) broth of plates with colonies | ||
| + | **pSB1AC3, pSB1AK3 | ||
| + | *Repeat assemblies | ||
| + | **Doing digestion according to the [http://openwetware.org/wiki/Knight:Restriction_Digest Knight protocol] now | ||
Latest revision as of 17:07, 9 July 2008
Overnight Results
- pSB1A10 screening plasmid grew just fine
- Assembly 1 (BP1 + Term) grew more colonies
- pSB1AC3 and pSB1AK3 both grew a small number of colonies
Goals
- Transform overnight ligations
- Note: Considering the other ligations didn't work and the restriction digest might be the problem we don't expect much
- Make small (10ml) broth of plates with colonies
- pSB1AC3, pSB1AK3
- Repeat assemblies
- Doing digestion according to the [http://openwetware.org/wiki/Knight:Restriction_Digest Knight protocol] now
