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Team:Hawaii/Notebook/2008-07- 9
From 2008.igem.org
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:* colony purification for downstream culture-upscaling and preparation of DB3.1 competent cells | :* colony purification for downstream culture-upscaling and preparation of DB3.1 competent cells | ||
:* DB3.1 strain will be used to accept plasmids containing the ccdB "death gene" | :* DB3.1 strain will be used to accept plasmids containing the ccdB "death gene" | ||
| + | :* Sm plate grew, plate lacking antibiotics grew, Kan plate did not grow (as expected) | ||
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| + | [[Image: | ||
===Run PCR Products on Gel=== | ===Run PCR Products on Gel=== | ||
Latest revision as of 03:41, 15 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Transformation of DH5a with synthetic oligonucleotide ligations
- Krystle
- Followed protocol used for transformation of cells with BioBrick plasmids from filter
- pilA, slr1, slr2 combined with homemade competent cells (batch 3)
- pnir combined with commercial supercompetent cells.
Streak Out DB3.1
- Krystle
- colony purification for downstream culture-upscaling and preparation of DB3.1 competent cells
- DB3.1 strain will be used to accept plasmids containing the ccdB "death gene"
- Sm plate grew, plate lacking antibiotics grew, Kan plate did not grow (as expected)
[[Image:
Run PCR Products on Gel
- Margaret
- PCR amplification of Omega cassette and OriR
- see here for updates for the pRL1383a amplification
Drylab Work
Wiki updates
- Grace
- Updated "Protocols" section with new protocols.
- Updated Initial Oligo Synthesis experiment
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
