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Team:KULeuven/10 July 2008
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(New page: < return to notebook 10.30-17h in labs and room 00.210 of the LBI<br> Present: all students (Nick and Stefanie are excused in the afternoon) * (Hopefully) F...) |
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10.30-17h in labs and room 00.210 of the LBI<br> | 10.30-17h in labs and room 00.210 of the LBI<br> | ||
| - | Present: all students (Nick and Stefanie | + | Present: all students (Nick and Stefanie were excused in the afternoon) |
* (Hopefully) Final workout of the project. See the figure below. | * (Hopefully) Final workout of the project. See the figure below. | ||
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** Pulse generator has also been redone, based upon the first system with a key and a T7 RNA polymerase. This time however, the key comes first this time. We hope that its half life is sufficiently short. | ** Pulse generator has also been redone, based upon the first system with a key and a T7 RNA polymerase. This time however, the key comes first this time. We hope that its half life is sufficiently short. | ||
** Modelling in CellDesigner: inverter (modified) + cell death (new), input-ouput (new), memory (new), filter (modified) + cell death (new), pulse generator (new) | ** Modelling in CellDesigner: inverter (modified) + cell death (new), input-ouput (new), memory (new), filter (modified) + cell death (new), pulse generator (new) | ||
| - | ** | + | ** [http://student.kuleuven.be/~s0158649/FinaalProject.ppt A presentation] has been prepared for next week to inform the advisors, instructors, students and other fanatics. |
[[image:Overview.jpg|center]] | [[image:Overview.jpg|center]] | ||
Revision as of 18:23, 10 July 2008
10.30-17h in labs and room 00.210 of the LBI
Present: all students (Nick and Stefanie were excused in the afternoon)
- (Hopefully) Final workout of the project. See the figure below.
- Smaller problems, we got rid of the miRNA again and decided to go for a complete antisense LuxI. This will be part of a new memory system that will allow HSL to accumulate if and only if an input signal has been received once in the past.
- We also inserted the TetR together with LuxR under control of a constitutive promotor.
- Most of the the Ribosome Binding Sites have been changed to less efficient ones in order to allow modification to weaker AND stronger ones if necessary.
- Pulse generator has also been redone, based upon the first system with a key and a T7 RNA polymerase. This time however, the key comes first this time. We hope that its half life is sufficiently short.
- Modelling in CellDesigner: inverter (modified) + cell death (new), input-ouput (new), memory (new), filter (modified) + cell death (new), pulse generator (new)
- [http://student.kuleuven.be/~s0158649/FinaalProject.ppt A presentation] has been prepared for next week to inform the advisors, instructors, students and other fanatics.
