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Minnesota/10 July 2008
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|NOTE: Total volume in dephosphorylation = 56.6uL | |NOTE: Total volume in dephosphorylation = 56.6uL | ||
Revision as of 16:05, 10 July 2008
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| 1. Dephosphorylation: Continued with double digestion base vector product from 07-09-2008. Used Antartic phosphoylase to dephosphorylate the base vector. Want to dephosphorylate the base vector to prevent it from closing - want it to stay a linear digested product. |
| Gene | 10x Buffer | DNA | Phosphotase |
|---|---|---|---|
| Base Vector | 5.6uL | 50.0uL | 1.0uL |
