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Team:UNIPV-Pavia/Notebook/Week7
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*Colony PCR for '''BBa_J23100'''-BBa_E0240 and '''BBa_B0030'''-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations. | *Colony PCR for '''BBa_J23100'''-BBa_E0240 and '''BBa_B0030'''-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations. | ||
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| + | |[[Image:pv_pcr_01_08.jpg|thumb|300px|left|Colony PCR for '''BBa_J23100'''-BBa_E0240, '''BBa_B0030'''-BBa_E0061: Marker and 10 colonies for each ligation]] | ||
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*We infected 9 ml LB + Amp with 30 µl of: | *We infected 9 ml LB + Amp with 30 µl of: | ||
Revision as of 15:36, 13 July 2008
 Home
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|  The Project
|  Biological Safety
|  Parts Submitted to the Registry
|
|---|---|---|---|---|
 Dry Lab
|  Wet Lab
|  Modeling
|  Protocols
|  Activity Notebook
|
Notebook
| Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 |
|---|
Week 5: 06/23/08 - 06/27/08
06/30/08
- We received sequencing results for BBa_B0030-BBa_C0061 (4th colony and 7th colony): sequences were correct! We decided to keep the 4th colony.
- Colony PCR for BBa_J23100-BBa_E0240 and BBa_B0030-BBa_C0061: 10 colonies for every plate. Gel showed many working colonies: we chose first colonies for the two ligations.
 |
- We infected 9 ml LB + Amp with 30 µl of:
| BBa_C0012 | BBa_B1006 | BBa_R0051-BBa_B0030 | BBa_B0030-BBa_C0061 (4) |
| BBa_C0062 | BBa_J23100-BBa_E0240 (1) | BBa_B0030-BBa_C0078 (8) | BBa_J23100-BBa_B0030 |
| BBa_C0040 | BBa_I15010 |
07/1/08
