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Virginia/14 July 2008
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(New page: ==Goals== *turn in time sheets! *Examine flourescent proteins under flourescence camera **psb1A10 **assembly 3 of i715039 and E0240 *make overnight broths of these flourescent protein cell...) |
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**our ligation glows red but our GFP E0240 has still never worked, we need to figure out whats going on with this part | **our ligation glows red but our GFP E0240 has still never worked, we need to figure out whats going on with this part | ||
*since RFP works we will ligate it with a known terminator to assemble with a GFP that we can prove works on its own | *since RFP works we will ligate it with a known terminator to assemble with a GFP that we can prove works on its own | ||
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Latest revision as of 14:46, 15 July 2008
Goals
- turn in time sheets!
- Examine flourescent proteins under flourescence camera
- psb1A10
- assembly 3 of i715039 and E0240
- make overnight broths of these flourescent protein cells and of the BP2 and BP3 genes
- assemble I715039 (RFP) with terminator B1002
Results
- while examining flourescent cells we discover a couple things
- psb1A10 needs to be induced by a plate with arabidose in the gel
- our ligation glows red but our GFP E0240 has still never worked, we need to figure out whats going on with this part
- since RFP works we will ligate it with a known terminator to assemble with a GFP that we can prove works on its own
