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Wisconsin: Lignin Project/17 June 2008
From 2008.igem.org
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Ran PCR product on 1% agrose gel; only ladder visible <br> | Ran PCR product on 1% agrose gel; only ladder visible <br> | ||
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| - | ''' | + | == Team Sorbitol == |
| + | |||
| + | Harvested the chromosomal DNA from the overnight MG1655 cultures. | ||
| + | |||
| + | Used this DNA as a template for 2 PCR reactions | ||
| + | |||
| + | One reaction was to clone out ''srlD'' the other was to clone the ''srl'' operon. | ||
| + | |||
| + | However when run on a gel no bands were found. | ||
Revision as of 19:47, 31 July 2008
Team Fungus:
Received primers
Diluted primers
Ran TAQ PCR reaction with Carbon-limited cDNA
Ran PCR product on 1% agrose gel; only ladder visible
Team Sorbitol
Harvested the chromosomal DNA from the overnight MG1655 cultures.
Used this DNA as a template for 2 PCR reactions
One reaction was to clone out srlD the other was to clone the srl operon.
However when run on a gel no bands were found.
