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Alberta NINT/29 May 2008
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| + | # colonies from K102010 ligation: ~100 negative ctl: 0 | ||
| + | setup 8 O/Ns | ||
Revision as of 21:43, 11 October 2008
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lab work (SD):
Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B)
(plates from 28/05/09).
DNA was run on 2% agarose gel. All 9 samples were identical on the gel.
K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were
selected at random, inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.
lab work (JD):
Gel purified R0011 band from gel electrophoresis.
Carried out quantitative analysis
Carried out ligation to create K102001 (R0011+T/A1)
Transformed XL1-B cells with K102001.
Prepared R0011 for sequencing and dropped it off.
lab work (WM):
# colonies from K102010 ligation: ~100 negative ctl: 0
setup 8 O/Ns
