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Wisconsin: Lignin Project/25 June 2008
From 2008.igem.org
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Ran the rest of digestion reaction out on another 1% agrose gel and purified using QIAquick gel extraction kit | Ran the rest of digestion reaction out on another 1% agrose gel and purified using QIAquick gel extraction kit | ||
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| - | + | == Team Sorbitol == | |
| + | |||
| + | Attended the Wisconsin iGEM team meeting | ||
| + | |||
| + | Re-evaluated our PCR reactions and primers | ||
| + | |||
| + | Decided to alter the temperatures and times of several steps. | ||
| + | |||
| + | Also we redesigned the primers used to clone out the ''srl'' operon. | ||
Revision as of 19:59, 31 July 2008
Team Fungus:
Digested PCR product and pET28a vector
Ran 1% agrose gel of digestion and performed gel purification
-digested and undigested PCR product as well as digested vector showed correctly sized bands
-undigested vector showed up at about 3kb when compared to supercoil ladder and at 4kb when compared to linear 1kb ladder
Ran the rest of digestion reaction out on another 1% agrose gel and purified using QIAquick gel extraction kit
Team Sorbitol
Attended the Wisconsin iGEM team meeting
Re-evaluated our PCR reactions and primers
Decided to alter the temperatures and times of several steps.
Also we redesigned the primers used to clone out the srl operon.
