Team:The University of Alberta/16 July 2008

From 2008.igem.org

(Difference between revisions)
(Today)
(Today)
Line 2: Line 2:
*The plates of Transformants of I0500 in the new vector turned out good
*The plates of Transformants of I0500 in the new vector turned out good
**Did colony PCR on two colonies to confirm they have the I0500 insert
**Did colony PCR on two colonies to confirm they have the I0500 insert
 +
***Ran products out on a 1% agarose gel
***Streaked out both colonies  
***Streaked out both colonies  
***Also made O/Ns of the colonies
***Also made O/Ns of the colonies

Revision as of 21:19, 16 July 2008

Today

  • The plates of Transformants of I0500 in the new vector turned out good
    • Did colony PCR on two colonies to confirm they have the I0500 insert
      • Ran products out on a 1% agarose gel
      • Streaked out both colonies
      • Also made O/Ns of the colonies
  • Transformations of the synthed biobricks worked!
    • Colony PCR to check the inserts
    • Set up O/Ns