Team:Hawaii/Notebook/2008-07-17

From 2008.igem.org

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== Drylab Work ==
 
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===Name of Task===
 
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:<strong> name of person/people who performed the task</strong>
 
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:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
 
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:* e.g. read through papers, worked on proposal, etc.
 

Revision as of 03:39, 18 July 2008

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Things we did today

Wetlab work

PCR

Grace
  • Colony PCR for GFP fusion brick and new pRL1383a to verify inserts
  • Sequencing (25 mu;l reactions)
  • nir promoter
  • slr2016
  • pilA
  • BBa_B0015
  • BBa_B0024
  • BBa_B0030
  • BBa_C0012
  • BBa_E0040
  • BBa_J04430
  • BBa_J33207
  • BBa_R0010
  • Ran all PCR products on a 4% gel to check
  • Gel ran funny -- loading dye ran 2x as fast as the DNA itself
  • Poor ladder resolution on gel
  • No bands seen for GFP fusion, pRL1383a, R0010
  • Incorrect bands for B0015 and J33207
  • Will redo plasmid preps for R0010, B0015, J33207
  • Will redo PCR reaction tomorrow for pRL1383a, GFP fusion, B0015, J33207, R0010

Plasmid Prep

Grace
  • Inoculated 7 ml LB + amp100 with single colony of B0015, R0010, J04430 for redo of plasmid prep tomorrow

Restreak to Purify

Grace
  • Restreaked GFP fusion and pRL1383a (MCS replaced) transformants to purify

pSB3K3 plasmid prep

Margaret


Discussion

Quote of the Day

History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson


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