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Team:University of Ottawa/18 July 2008
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::<li> For miniprep on Monday, I think I will choose 5 colonies per plate. | ::<li> For miniprep on Monday, I think I will choose 5 colonies per plate. | ||
::<li> An inoculation will be performed today into liquid media so that a miniprep can be performed. | ::<li> An inoculation will be performed today into liquid media so that a miniprep can be performed. | ||
| + | '''Dan''' | ||
| + | :'''PCR''' | ||
| + | ::<li> PCR amplification of 0B and 1 A was performed today, absorbance measurements indicated decent concentrations | ||
| + | :'''Digestion''' | ||
| + | ::<li>1A 0B and T were pooled and digested with PstI and SphI as part of our new strategy for designing the constructs. It should help eliminate virtually all possible byproducts. | ||
| + | :'''Ligation''' | ||
| + | ::<li> Was performed overnight. | ||
Revision as of 14:52, 22 July 2008
Contents |
Today in the Lab
Matt
- PCR Amplification
- A PCR Amplification was performed on 600/1 plasmids with integration primers so that yeast integration can be performed next week. Corey suggested it is better to make new stock of this PCR amplification product for integration.
- Transformation
- The transformation was a success with both plates streaked growing numerous colonies.
- For miniprep on Monday, I think I will choose 5 colonies per plate.
- An inoculation will be performed today into liquid media so that a miniprep can be performed.
Dan
- PCR
- PCR amplification of 0B and 1 A was performed today, absorbance measurements indicated decent concentrations
- Digestion
- 1A 0B and T were pooled and digested with PstI and SphI as part of our new strategy for designing the constructs. It should help eliminate virtually all possible byproducts.
- Ligation
- Was performed overnight.
