Team:Hawaii/Project

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Overall Project

Cyanobacteria are frequently studied for their ability to harness the power of photosynthesis in order to produce a wide variety of useful products including bio-fuels and -polymers. Such tasks are accomplished by these ‘little green factories’ with a minimal input of salts, light, and carbon dioxide. We aim to expand the availability of BioBrick vectors to cyanobacteria in order to “open source” the current BioBrick registry to a greater range of organisms.

We plan to engineer:

1) a mobilizable broad-host-range BioBrick vector that can be used to transfer genetic information between E. coli and Synechocystis sp. 6803, with the future possibility of transforming plants via Agrobacteria;

2) a cassette for protein export from Synechocystis; and

3) the nitrate-inducible cyanobacterial nir promoter.

The functionality of the parts we engineer will be demonstrated by achieving inducible protein production and export of a lichenase and a GFP construct introduced into Synechocystis using our novel BioBrick shuttle vector.

Project Details

Part A: Mobilizable Broad-Host-Range Plasmid

Part B: Cyanobacterial protein secretion system

Results

[http://manoa.hawaii.edu/ ][http://manoa.hawaii.edu/ovcrge/ ][http://www.ctahr.hawaii.edu ]

@@ Line 2: / Line 2: @@
 == Overall Project ==
-Cyanobacteria are frequently studied for their abilities to photosynthesize and produce a wide variety of useful products including biofuels and biopolymers. These photoautotrophs, however, have yet to be fully exploited as biotechnological tools due to the lack of broad host range vectors between cyanobacteria and model organisms such as ''E. coli''. By expanding the availability of BioBrick vectors to cyanobacteria, we can “open source” the current BioBrick registry to a greater range of organisms. Furthermore, we will effectively create mini “green factories,” capable of using only light, water, and carbon dioxide to synthesize biomolecules of interest.
+Cyanobacteria are frequently studied for their ability to harness the power of photosynthesis in order to produce a wide variety of useful products including bio-fuels and -polymers. Such tasks are accomplished by these ‘little green factories’ with a minimal input of salts, light, and carbon dioxide. We aim to expand the availability of BioBrick vectors to cyanobacteria in order to “open source” the current BioBrick registry to a greater range of organisms.
 <br><br>
 We plan to engineer:<br>
@@ Line 8: / Line 8: @@
 : 2) a cassette for protein export from ''Synechocystis''; and<br>
 : 3) the nitrate-inducible cyanobacterial nir promoter.  <br><br>
-In addition, we plan to convert the omega interposon, which confers streptomycin and spectinomycin resistance and has proven useful for insertional gene inactivation, into BioBrick format. The functionality of the parts we engineer will be demonstrated by achieving inducible protein production and export of a lichenase and a GFP construct introduced into ''Synechocystis'' using our novel BioBrick shuttle vector.
+The functionality of the parts we engineer will be demonstrated by achieving inducible protein production and export of a lichenase and a GFP construct introduced into ''Synechocystis'' using our novel BioBrick shuttle vector.
 == Project Details==