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Team:ESBS-Strasbourg/21 July 2008
From 2008.igem.org
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* Overnight culture of p1010 A2 (LB+Amp) and p1010 K3 (LB+Kan), i.e. 200µl of little overnight culture in 100 ml medium | * Overnight culture of p1010 A2 (LB+Amp) and p1010 K3 (LB+Kan), i.e. 200µl of little overnight culture in 100 ml medium | ||
* Overnight culture of Top10 cells from Invitrogen (cultivated on a plate) to make them competent tomorrow | * Overnight culture of Top10 cells from Invitrogen (cultivated on a plate) to make them competent tomorrow | ||
| - | + | * PCR of VP16 (3 different templates) Tup1,LacI,CI,TetR @ 48,50,52,53 °C -> none worked | |
== General == | == General == | ||
== Quote of the day == | == Quote of the day == | ||
Latest revision as of 10:50, 24 July 2008
Contents |
DryLab
Modeling
WetLab
- Miniprep of TetR (MM)
- Overnight culture of p1010 A2 (LB+Amp) and p1010 K3 (LB+Kan), i.e. 200µl of little overnight culture in 100 ml medium
- Overnight culture of Top10 cells from Invitrogen (cultivated on a plate) to make them competent tomorrow
- PCR of VP16 (3 different templates) Tup1,LacI,CI,TetR @ 48,50,52,53 °C -> none worked
