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Team:Hawaii/Notebook/2008-07-21
From 2008.igem.org
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| - | :* | + | :* We figured out why some of the biobrick extractions did not transform. Some of these parts are on pSB2K3[[http://partsregistry.org/wiki/index.php?title=Part:pSB2K3]] which is a low copy until IPTG is added to the medium in which case it becomes high copy! So next time we make this, add 1mM IPTG to the media. |
| - | : | + | |
Revision as of 04:23, 22 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Colony PCR
- Margaret
- Colony PCR of pSB1A2, pSB1A3, pSB3K3
PCR amplification of pRL1383a parts
- continued from Saturday, ran a gel
Restriction Digest
- Krystle
- Performed 2 restriction digests on nir, gfp, and gfp fusion brick with EcoRI and SpeI
- trial 1: digested 5ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 3 hours
- trial 2: digested 25ul of each plasmid prep in a 50ul reaction mixture, ran 20 ul of the digest after 2 hours
Drylab Work
Name of Task
- Krystle, Margaret
- We figured out why some of the biobrick extractions did not transform. Some of these parts are on pSB2K3http://partsregistry.org/wiki/index.php?title=Part:pSB2K3 which is a low copy until IPTG is added to the medium in which case it becomes high copy! So next time we make this, add 1mM IPTG to the media.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
