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Revision as of 18:32, 22 July 2008

PCR products PCR products Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date [[ ]] [[ ]] Plasmids: MiniPrep Products Plasmids: MiniPrep Products Number Name Biobricks Description Products in use Date MP1 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Strong constitutive promoter in J61002 (clones 1 + 2) 2007 MP2 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Medium constitutive promoter in J61002 (clones 1 + 2) MP2.2 2007 MP3 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] Double terminator (clones 1 + 2) MP3.3, MP3.4 2007 MP4 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] AraC pBAD (clones 1 + 2) 2007 MP5 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Strong RBS (clones 1 + 2) 2007 MP6 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] ECFP (RBS+LVA+Term) (clones 1 + 2) MP6'.1, MP6'.2 2007 MP8 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] gfp tri-part; strong rbs (clones 1 + 2) 2007 Plasmids: MiniPrep "in preparation" Number Name Biobricks Description Products in use Date [http://partsregistry.org/Part:BBa_B0034 B0034] Strongest RBS 2008 [http://partsregistry.org/Part:BBa_C0040 C0040] tetR repressor 2008 [http://partsregistry.org/Part:BBa_C0079 C0079] lasR activator with LVA Tag 2008 [http://partsregistry.org/Part:BBa_C0179 C0179] lasR activator 2007 [http://partsregistry.org/Part:BBa_E0030 E0030] YFP 2008 [http://partsregistry.org/Part:BBa_E0040 E0040] GFP 2008 [http://partsregistry.org/Part:BBa_E1010 E1010] mRFP 2008 [http://partsregistry.org/Part:BBa_J23101 J23101] Strongest Constitutive promoter 2008 [http://partsregistry.org/Part:BBa_J23109 J23109] Weak Constitutive promoter 2008 [http://partsregistry.org/Part:BBa_R0040 R0040] tetR repressible promoter 2008 [http://partsregistry.org/Part:BBa_R0079 R0079] promoter pLas (lasr & PAI regulated) 2008 [http://partsregistry.org/Part:BBa_S03154 S03154] B0034(rbs) ->LasI 2008 [http://partsregistry.org/Part:BBa_S03879 S03879] B0034(rbs) -> TetR 2008 Digestion Products PURPLE = Vector GREEN = Insert Digestion Products Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description Product Left []ng/µL Date D1 <bbpart>pJ23100</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 2096 Vector with the J23100 promoter ready for insertion of a RBS/CDS insert Ø (24/09/07) 34 July 17 D2 <bbpart>mRFP dblTer</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 887 27 July 17

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