Team:Paris/Notebook/Freezer

From 2008.igem.org

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== Strains (glycerol stock) ==
 +
{|
 +
|- style="background: #ccccff;"
 +
! colspan="5" style="background: #ccccff;" | Strains glycerol stock
 +
|- style="background: #ccccff; text-align: center;"
 +
|width=5%| Number
 +
|width=25%| Name
 +
|width=55%| Description
 +
|width=5%| Verified clone
 +
|width=5%| Date
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S1
 +
|MG1655
 +
|Standard E.Coli strain
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S2
 +
|Acinetobacter ADP1
 +
|Strain used for TG coloration test with Red Nile
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S3
 +
|DH5alpha pKs::DGAT
 +
|Enzyme catalysing the transormation of DAG into TG
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S4
 +
|<bbpart>BBa_pJ23100</bbpart>
 +
|Strong constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23100 SpecifiedComponents</partinfo>
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S5
 +
|<bbpart>BBa_B0015</bbpart>
 +
|Double terminator (clones 1 + 2) <partinfo>B0015 SpecifiedComponents</partinfo>
 +
|
 +
|
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S6
 +
|<bbpart>BBa_pJ23107</bbpart>
 +
|Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23107 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 19| July 19]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S7
 +
|<bbpart>BBa_B0030</bbpart>
 +
|Strong RBS (clones 1 + 2) <partinfo>B0030 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 19| July 19]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S8
 +
|<bbpart>BBa_E0422</bbpart>
 +
|ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 19| July 19]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S9
 +
|<bbpart>BBa_E0241</bbpart>
 +
|PoPs to GFP converter (clones 1 + 2) <partinfo>E0241 DeepComponents</partinfo>
 +
|
 +
|[[Paris/July 19| July 19]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S10
 +
|<bbpart>BBa_E0840</bbpart>
 +
|gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 19| July 19]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S11
 +
|<bbpart>BBa_J61047</bbpart>
 +
|Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 19| July 19]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S12
 +
|W121
 +
|
 +
|
 +
|05/07/07
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S13
 +
|<bbpart>BBa_I0500</bbpart>
 +
|pBAD AraC (clone 1+2) <partinfo>I0500 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 20| July 20]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S14
 +
|<bbpart>BBa_pJ23107</bbpart>
 +
|Medium constitutive promoter in J61002 (clone 3+4) <partinfo>pJ23107 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 20| July 20]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S15
 +
|<bbpart>BBa_P1003</bbpart>
 +
|Kanamycin resistance cassette (clone 1+2) <partinfo>P1003 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 20| July 20]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S16
 +
|<bbpart>J61002</bbpart>::<bbpart>I0500</bbpart> 
 +
|AraC pBad regulating mRFP expression in pJ61002 (L1) <partinfo>J61002 SpecifiedComponents</partinfo>
 +
|L1.2, L1.3
 +
|[[Paris/July 27| July 27]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S17
 +
|<bbpart>pSB1A2</bbpart>::Lox66-DapA Coli
 +
|Lox66-DapA Coli cloned as a biobrick (L2)
 +
|
 +
|[[Paris/July 27| July 27]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S18
 +
|<bbpart>pSB1A2</bbpart>::B0030 + J61047
 +
|RBS(B0030)-Cre ORF (L6) (clones 1+2)
 +
|
 +
|[[Paris/July 27| July 27]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S19
 +
|<bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37026</bbpart>
 +
|Lox66 (L9)(1 clone) <partinfo>J37026 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 27| July 27]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S20
 +
|<bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37028</bbpart>
 +
|Lox71 (L10)(1 clone) <partinfo>J37028 SpecifiedComponents</partinfo>
 +
|
 +
|[[Paris/July 27| July 27]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S21
 +
|<bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37028</bbpart>+<bbpart>BBa_E0840</bbpart>
 +
|Lox71-gfp tri-part (L12)
 +
|L12.2 ok
 +
|?
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S22
 +
|<bbpart>pSB1A2</bbpart>::Lox71-kmR-lox66
 +
|Lox71-kmR-lox66 (L25)
 +
|sequence not good (thrown away)
 +
|?
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S23
 +
|pTopo::P5
 +
|Lox66-DapAColi
 +
|
 +
|?
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S24
 +
|pTopo::P6
 +
|Lox66-DapASubtilis
 +
|
 +
|?
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S25
 +
|pTopo::P8
 +
|RBS-DapAColi
 +
|MPT4.3, MPT4.5
 +
|?
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S26
 +
|pTopo::P9
 +
|RBS-DapASubtilis
 +
|
 +
|?
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S27
 +
|<bbpart>J61002</bbpart>
 +
|
 +
|
 +
|[[Paris/August 14| August 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S28
 +
|<bbpart>J61025</bbpart>
 +
|FRT-Cm-FRT
 +
|
 +
|[[Paris/August 14| August 14]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S29
 +
|L26
 +
|Insertion of RBS-Cre behind araC/pBad
 +
|
 +
|[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S30
 +
|L27
 +
|Insertion of ftsZ behind araC/pBad
 +
|
 +
|[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S31
 +
|L28
 +
|Insertion of ftsAZ behind araC/pBad
 +
|
 +
|[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S32
 +
|L29
 +
|Insertion of gfp-tripart in front of lox66
 +
|
 +
|[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S33A
 +
|L30A
 +
|cloning dapAp promotor in J61002
 +
|
 +
|[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S33B
 +
|L30B
 +
|cloning dapAp promotor in J61002
 +
|
 +
|[[Paris/August 13| August 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S34
 +
|L31
 +
|Insertion of B0015 terminator behind lox71
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S35
 +
|L32
 +
|Insertion of B0015 terminator in front of lox66
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S36
 +
|L34
 +
|pTet>>lox71-gfptripart
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S37
 +
|L35
 +
|araC/pBad>>lox71-gfptripart
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S38
 +
|L36
 +
|lox66-mRFP
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S39
 +
|L37
 +
|lox66-RBS-dapAcoli BI
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S40
 +
|L39
 +
|RBS-dapAcoli cloned in pSB1A2
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S41
 +
|L40
 +
|RBS-dapAsubtilis cloned in pSB1A2
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S42
 +
|L41
 +
|lox71-ftsZ cloned in pSB1A2
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S43
 +
|L42
 +
|lox71-ftsA-Z cloned in pSB1A2
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S44
 +
|L43
 +
|RBS-dapAsubtilis cloned in pSB1A2
 +
|
 +
|[[Paris/August 22| August 22]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S45A
 +
|L30A
 +
|DapAp>>mRFP within W121 strain
 +
|
 +
|[[Paris/August 25| August 25]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S45B
 +
|L30B
 +
|DapAp>>mRFP within W121 strain
 +
|
 +
|[[Paris/August 25| August 25]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S48
 +
|pKD13
 +
|FRT-KanR-FRT; Wanner plasmid
 +
|
 +
|[[Paris/September 18| Sep. 18]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S49
 +
|pKD46
 +
|Red recombination system; Wanner plasmid
 +
|
 +
|[[Paris/September_13| Sep. 13]]
 +
|- style="background: #cccccc;" 
 +
| style="background: #ccffcc;" |S50
 +
|L30 plasmid in FR781
 +
|dapAp>>mRFP plasmid
 +
|
 +
|[[Paris/September_28| Sep. 28]]
 +
|}
 +
 +
 +
* p2C (CFP fluorescent)
 +
* p2Y (YFP fluorescent)
 +
* FtsZ<sup>TS</sup> strains
 +
** MG1655 FtsZ -TS 84
 +
** 121.1
 +
** 121.2
 +
** 121.3
 +
** 121.4
 +
** 121.5
 +
** 121.6
 +
** 129
 +
** 1.129
 +
** DCR 14.1
 +
** DCR 14.2
 +
** DCR 14.3

Revision as of 10:11, 23 July 2008

Contents

PCR products

PCR products
Number Name Oligo F Oligo R Matrix Length Comments Product Left ? []ng/µL Date
[[ ]]
[[ ]]


Plasmids: MiniPrep Products

Plasmids: MiniPrep Products
Number Name Biobricks Description Products in use Date
MP1 [http://partsregistry.org/Part:BBa_J23100 pJ23100] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Strong constitutive promoter in J61002 2007
MP2 [http://partsregistry.org/Part:BBa_J23107 pJ23107] in [http://partsregistry.org/Part:BBa_J61002 J61002] Part icon regulatory.png Medium constitutive promoter in J61002 MP2.2 2007
MP3 [http://partsregistry.org/Part:BBa_B0015 B0015] in [http://partsregistry.org/Part:BBa_SB1AK3 pSB1AK3] Part icon terminator.pngPart icon terminator.png Double terminator MP3.3, MP3.4 2007
MP4 [http://partsregistry.org/Part:BBa_I0500 I0500] in [http://partsregistry.org/Part:BBa_SB2K3 pSB2K3] Part icon regulatory.png AraC pBAD 2007
MP5 [http://partsregistry.org/Part:BBa_B0030 B0030] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.png Strong RBS (Efficiency = 0,6) 2007
MP6 [http://partsregistry.org/Part:BBa_E0422 E0422] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png ECFP (RBS+LVA+Term) MP6'.1, MP6'.2 2007
MP8 [http://partsregistry.org/Part:BBa_E0840 E0840] in [http://partsregistry.org/Part:BBa_SB1A2 pSB1A2] Part icon rbs.pngPart icon reporter.pngPart icon terminator.png gfp tri-part; strong rbs 2007


Plasmids: MiniPrep "in preparation"
Number Name Biobricks Description Products in use Date
[http://partsregistry.org/Part:BBa_B0034 B0034] Part icon rbs.png Strongest RBS (Efficiency = 1) 2008
[http://partsregistry.org/Part:BBa_C0040 C0040] Icon coding.png tetR repressor 2008
[http://partsregistry.org/Part:BBa_C0079 C0079] Icon coding.png lasR activator with LVA Tag 2008
[http://partsregistry.org/Part:BBa_C0179 C0179] Icon coding.png lasR activator 2007
[http://partsregistry.org/Part:BBa_E0030 E0030] Part icon reporter.png YFP 2008
[http://partsregistry.org/Part:BBa_E0040 E0040] Part icon reporter.png GFP 2008
[http://partsregistry.org/Part:BBa_E1010 E1010] Part icon reporter.png mRFP 2008
[http://partsregistry.org/Part:BBa_J23101 J23101] Part icon regulatory.png Strongest Constitutive promoter 2008
[http://partsregistry.org/Part:BBa_J23109 J23109] Part icon regulatory.png Weak Constitutive promoter 2008
[http://partsregistry.org/Part:BBa_R0040 R0040] Part icon regulatory.png tetR repressible promoter 2008
[http://partsregistry.org/Part:BBa_R0079 R0079] Part icon regulatory.png promoter pLas (lasr & PAI regulated) 2008
[http://partsregistry.org/Part:BBa_S03154 S03154] Part icon rbs.pngIcon coding.png B0034(rbs) ->LasI 2008
[http://partsregistry.org/Part:BBa_S03879 S03879] Part icon rbs.pngIcon coding.png B0034(rbs) -> TetR 2008

Digestion Products

(Legend: Vector, Insert)

Digestion Products
Number Product Name Matrix Name Enzyme 1 Enzyme 2 Size Description Product Left []ng/µL Date
D1 <bbpart>pJ23100</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 2096 Vector with the J23100 promoter ready for insertion of a RBS/CDS insert Ø (24/09/07) 34 July 17
D2 <bbpart>mRFP dblTer</bbpart> <bbpart>pJ23100</bbpart> Spe1 Pst1 887 27 July 17


Strains (glycerol stock)

Strains glycerol stock
Number Name Description Verified clone Date
S1 MG1655 Standard E.Coli strain
S2 Acinetobacter ADP1 Strain used for TG coloration test with Red Nile
S3 DH5alpha pKs::DGAT Enzyme catalysing the transormation of DAG into TG
S4 <bbpart>BBa_pJ23100</bbpart> Strong constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23100 SpecifiedComponents</partinfo>
S5 <bbpart>BBa_B0015</bbpart> Double terminator (clones 1 + 2) <partinfo>B0015 SpecifiedComponents</partinfo>
S6 <bbpart>BBa_pJ23107</bbpart> Medium constitutive promoter in J61002 (clones 1 + 2) <partinfo>pJ23107 SpecifiedComponents</partinfo> July 19
S7 <bbpart>BBa_B0030</bbpart> Strong RBS (clones 1 + 2) <partinfo>B0030 SpecifiedComponents</partinfo> July 19
S8 <bbpart>BBa_E0422</bbpart> ECFP (RBS+LVA+Term) (clones 1 + 2) <partinfo>E0422 SpecifiedComponents</partinfo> July 19
S9 <bbpart>BBa_E0241</bbpart> PoPs to GFP converter (clones 1 + 2) <partinfo>E0241 DeepComponents</partinfo> July 19
S10 <bbpart>BBa_E0840</bbpart> gfp tri-part; strong rbs (clones 1 + 2) <partinfo>E0840 SpecifiedComponents</partinfo> July 19
S11 <bbpart>BBa_J61047</bbpart> Cre ORF (clones 1 + 2) <partinfo>J61047 SpecifiedComponents</partinfo> July 19
S12 W121 05/07/07
S13 <bbpart>BBa_I0500</bbpart> pBAD AraC (clone 1+2) <partinfo>I0500 SpecifiedComponents</partinfo> July 20
S14 <bbpart>BBa_pJ23107</bbpart> Medium constitutive promoter in J61002 (clone 3+4) <partinfo>pJ23107 SpecifiedComponents</partinfo> July 20
S15 <bbpart>BBa_P1003</bbpart> Kanamycin resistance cassette (clone 1+2) <partinfo>P1003 SpecifiedComponents</partinfo> July 20
S16 <bbpart>J61002</bbpart>::<bbpart>I0500</bbpart> AraC pBad regulating mRFP expression in pJ61002 (L1) <partinfo>J61002 SpecifiedComponents</partinfo> L1.2, L1.3 July 27
S17 <bbpart>pSB1A2</bbpart>::Lox66-DapA Coli Lox66-DapA Coli cloned as a biobrick (L2) July 27
S18 <bbpart>pSB1A2</bbpart>::B0030 + J61047 RBS(B0030)-Cre ORF (L6) (clones 1+2) July 27
S19 <bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37026</bbpart> Lox66 (L9)(1 clone) <partinfo>J37026 SpecifiedComponents</partinfo> July 27
S20 <bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37028</bbpart> Lox71 (L10)(1 clone) <partinfo>J37028 SpecifiedComponents</partinfo> July 27
S21 <bbpart>pSB1A2</bbpart>::<bbpart>BBa_J37028</bbpart>+<bbpart>BBa_E0840</bbpart> Lox71-gfp tri-part (L12) L12.2 ok ?
S22 <bbpart>pSB1A2</bbpart>::Lox71-kmR-lox66 Lox71-kmR-lox66 (L25) sequence not good (thrown away) ?
S23 pTopo::P5 Lox66-DapAColi ?
S24 pTopo::P6 Lox66-DapASubtilis ?
S25 pTopo::P8 RBS-DapAColi MPT4.3, MPT4.5 ?
S26 pTopo::P9 RBS-DapASubtilis ?
S27 <bbpart>J61002</bbpart> August 14
S28 <bbpart>J61025</bbpart> FRT-Cm-FRT August 14
S29 L26 Insertion of RBS-Cre behind araC/pBad August 13
S30 L27 Insertion of ftsZ behind araC/pBad August 13
S31 L28 Insertion of ftsAZ behind araC/pBad August 13
S32 L29 Insertion of gfp-tripart in front of lox66 August 13
S33A L30A cloning dapAp promotor in J61002 August 13
S33B L30B cloning dapAp promotor in J61002 August 13
S34 L31 Insertion of B0015 terminator behind lox71 August 22
S35 L32 Insertion of B0015 terminator in front of lox66 August 22
S36 L34 pTet>>lox71-gfptripart August 22
S37 L35 araC/pBad>>lox71-gfptripart August 22
S38 L36 lox66-mRFP August 22
S39 L37 lox66-RBS-dapAcoli BI August 22
S40 L39 RBS-dapAcoli cloned in pSB1A2 August 22
S41 L40 RBS-dapAsubtilis cloned in pSB1A2 August 22
S42 L41 lox71-ftsZ cloned in pSB1A2 August 22
S43 L42 lox71-ftsA-Z cloned in pSB1A2 August 22
S44 L43 RBS-dapAsubtilis cloned in pSB1A2 August 22
S45A L30A DapAp>>mRFP within W121 strain August 25
S45B L30B DapAp>>mRFP within W121 strain August 25
S48 pKD13 FRT-KanR-FRT; Wanner plasmid Sep. 18
S49 pKD46 Red recombination system; Wanner plasmid Sep. 13
S50 L30 plasmid in FR781 dapAp>>mRFP plasmid Sep. 28


  • p2C (CFP fluorescent)
  • p2Y (YFP fluorescent)
  • FtsZTS strains
    • MG1655 FtsZ -TS 84
    • 121.1
    • 121.2
    • 121.3
    • 121.4
    • 121.5
    • 121.6
    • 129
    • 1.129
    • DCR 14.1
    • DCR 14.2
    • DCR 14.3
Retrieved from "http://2008.igem.org/Team:Paris/Notebook/Freezer"