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Team:Hawaii/Notebook/2008-07-22
From 2008.igem.org
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Revision as of 03:12, 23 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Alkaline Lysis Mini-prep
- Margaret
- Plasmid prep of pSB1A2, pSB1A3, and pSB3K3: 3 1.5 mL preps of the first two, and 10 of the third because it has a lower copy number. Used the protocol from Dr. Callahan's lab (http://packrat.stjohn.hawaii.edu/prestinglab/wiki/Protocols).
- I had to skip the phenol/chloroform step, so after adding Rnase A and incubating for 20 minutes, I spun down again, collected the supernatant and then precipitated with isopropanol.
- Grace
- nir, pilA, slr-1, slr-2, GFP fusion
Glycerol Stock
- Margaret
- glycerol stock of pSB1A2, pSB1A3, and pSB3K3. These DB3.1 strains containing these plasmids can be found in the -80C with the other plasmids in E. coli.
- Grace
- nir, pilA, slr-1, slr-2, GFP fusion, BB-pRL1383a, BBa_E0026
pRL1383a insert Clean-up
- in preparation for constructing the biobrick form of pRL1383a, cleaned the PCR products: mob, rep, oriV, and the aadA region using the "Isopropanol Precipitation for PCR purification" protocol from openwetware.org
- Cleaned the oriT construct using the Qiagen Minelute Gel Purification kit.
Drylab Work
Name of Task
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
