Team:Johns Hopkins/Notebook/GROUP 6: Vectors
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== GROUP 6: Vectors == | == GROUP 6: Vectors == | ||
- | Status report by | + | Status report by Tejas on 8/5/08 |
- | Vector transformed into bacteria strain DB3.1 | + | Vector transformed into bacteria strain DB3.1: No (currently being done) |
- | Permanent culture made in the Boeke lab for future reference | + | Permanent culture made in the Boeke lab for future reference: No |
- | Selectable marker for this vector | + | Selectable marker for this vector: (Amp, Kan, Cam) |
- | Medium made and tested | + | Medium made and tested: No (link) |
- | DNA preps made | + | DNA preps made: Yes |
- | DNA preps tested by RE digest | + | DNA preps tested by RE digest: Yes (link) |
DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109) – put data as | DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109) – put data as | ||
Table on moodle. | Table on moodle. |
Revision as of 21:04, 5 August 2008
GROUP 6: Vectors
Status report by Tejas on 8/5/08 Vector transformed into bacteria strain DB3.1: No (currently being done) Permanent culture made in the Boeke lab for future reference: No Selectable marker for this vector: (Amp, Kan, Cam) Medium made and tested: No (link) DNA preps made: Yes DNA preps tested by RE digest: Yes (link) DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109) – put data as Table on moodle. Sample format/data follows:
Amount transformed cfu/micGm in DB3.1 cfu/micGm in JM109 0.1 ng 5 * 10e7 <2 * 10e2
Preparative digests ready for use are located – where?
[http://www.jhu.edu/iGEM/Group6:Vectors/2008-7-24.Vectors%20(CAM,AMP,KAN3,KAN4)%20after%20Digest.Tejas.html Vectors (CAM,AMP,KAN3,KAN4) after Digest]