Team:Johns Hopkins/Notebook/GROUP 6: Vectors

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(GROUP 6: Vectors)
(GROUP 6: Vectors)
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== GROUP 6: Vectors ==
== GROUP 6: Vectors ==
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   Status report by ____
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   Status report by Tejas on 8/5/08
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   Vector transformed into bacteria strain DB3.1 Y/N
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   Vector transformed into bacteria strain DB3.1: No (currently being done)
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   Permanent culture made in the Boeke lab for future reference Y/N
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   Permanent culture made in the Boeke lab for future reference: No
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   Selectable marker for this vector
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   Selectable marker for this vector: (Amp, Kan, Cam)
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   Medium made and tested Y/N  (link)
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   Medium made and tested: No (link)
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   DNA preps made Y/N
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   DNA preps made: Yes
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   DNA preps tested by RE digest - (link)
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   DNA preps tested by RE digest: Yes (link)
   DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109) – put data as  
   DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109) – put data as  
     Table on moodle.   
     Table on moodle.   

Revision as of 21:04, 5 August 2008

GROUP 6: Vectors

 Status report by Tejas on 8/5/08
 Vector transformed into bacteria strain DB3.1: No (currently being done)
 Permanent culture made in the Boeke lab for future reference: No
 Selectable marker for this vector: (Amp, Kan, Cam)
 Medium made and tested: No (link)
 DNA preps made: Yes
 DNA preps tested by RE digest: Yes (link)
 DNA preps tested by transformation into DB3.1 and DH5alpha (or JM109) – put data as 
   Table on moodle.  
 Sample format/data follows:
Amount transformed cfu/micGm in DB3.1 cfu/micGm in JM109 0.1 ng 5 * 10e7 <2 * 10e2
Preparative digests ready for use are located – where?


 [http://www.jhu.edu/iGEM/Group6:Vectors/2008-7-24.Vectors%20(CAM,AMP,KAN3,KAN4)%20after%20Digest.Tejas.html Vectors (CAM,AMP,KAN3,KAN4) after Digest]