Team:University of Ottawa/23 July 2008

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__TOC__
__TOC__
==Today in the lab==
==Today in the lab==

Revision as of 18:30, 30 July 2008

Untitled Document

 

 


Contents

Today in the lab

Dan

PCR amplification of T123
  • I wanted to do PCR amplification of 0A and 0B, however PCR blocks were full, I will do this tomorrow
  • 6 tubes of PCR reaction were prepared for T123, hopefuly this will give me the amount of DNA that I need for a successful ligation.
    • Transformation of p2S and p2D
  • p2S and p2D were transformed in E. coli

    • Chris

    Minipreparation of AtCRE
  • Used minprep kit and protocol to isolate AtCRE from inoculated cells.
  • Measured absorbance of resulting DNA samples to determine concentrations
    • Confirmation of AtCRE
  • Performed a digestion of AtCRE with EcoRI against a water control and a positive control (DQ2325601 digested with EcoRI)
  • Ran product on a 1% gel for 40 minutes at 80V
  • Two of six samples showed desired bands.
    • Retrieved from "http://2008.igem.org/Team:University_of_Ottawa/23_July_2008"