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Team:The University of Alberta/28 July 2008
From 2008.igem.org
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**streaking for SCIs | **streaking for SCIs | ||
**set up O/Ns for minipreps tomorrow | **set up O/Ns for minipreps tomorrow | ||
| + | '''UPDATE''': Colony PCR showed products of the wrong size; all three were less than 650bp and should be ~1500bp. This means the plates and O/Ns are no good. Will redo with different colonies tomorrow; if I get wrong results again then I'll retransform the ligation. | ||
Latest revision as of 23:54, 28 July 2008
To Do
1. Religate TetR to RBS (assuming there are digests still available)
2. Resequence the TetR and RBS stocks
3. Obtain p0380 vector and begin site directed mutagenesis
4. Clean pots and plant more plants
5. Harvest seeds
6. Sequence J6+tryp transformants
Today
- The transformants of Tryp in J6 done on Friday turned out well. Picked 3 colonies and did:
- colony PCR
- streaking for SCIs
- set up O/Ns for minipreps tomorrow
UPDATE: Colony PCR showed products of the wrong size; all three were less than 650bp and should be ~1500bp. This means the plates and O/Ns are no good. Will redo with different colonies tomorrow; if I get wrong results again then I'll retransform the ligation.
