Team:University of Chicago/Notebook/Plasmids
From 2008.igem.org
(New page: ==pBluescript SK== [http://www.addgene.org/pgvec1?f=v&cmd=showvecinfo&vectorid=5495 Plasmid map source information]) |
(→pBluescript SK) |
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Line 1: | Line 1: | ||
==pBluescript SK== | ==pBluescript SK== | ||
[http://www.addgene.org/pgvec1?f=v&cmd=showvecinfo&vectorid=5495 Plasmid map source information] | [http://www.addgene.org/pgvec1?f=v&cmd=showvecinfo&vectorid=5495 Plasmid map source information] | ||
+ | |||
+ | ====Sequence==== | ||
+ | 1 | ||
+ | CACCTAAATT | ||
+ | GTAAGCGTTA | ||
+ | ATATTTTGTT | ||
+ | AAAATTCGCG | ||
+ | TTAAATTTTT | ||
+ | GTTAAATCAG | ||
+ | 61 | ||
+ | CTCATTTTTT | ||
+ | AACCAATAGG | ||
+ | CCGAAATCGG | ||
+ | CAAAATCCCT | ||
+ | TATAAATCAA | ||
+ | AAGAATAGAC | ||
+ | 121 | ||
+ | CGAGATAGGG | ||
+ | TTGAGTGTTG | ||
+ | TTCCAGTTTG | ||
+ | GAACAAGAGT | ||
+ | CCACTATTAA | ||
+ | AGAACGTGGA | ||
+ | 181 | ||
+ | CTCCAACGTC | ||
+ | AAAGGGCGAA | ||
+ | AAACCGTCTA | ||
+ | TCAGGGCGAT | ||
+ | GGCCCACTAC | ||
+ | GTGAACCATC | ||
+ | 241 | ||
+ | ACCCTAATCA | ||
+ | AGTTTTTTGG | ||
+ | GGTCGAGGTG | ||
+ | CCGTAAAGCA | ||
+ | CTAAATCGGA | ||
+ | ACCCTAAAGG | ||
+ | 301 | ||
+ | GAGCCCCCGA | ||
+ | TTTAGAGCTT | ||
+ | GACGGGGAAA | ||
+ | GCCGGCGAAC | ||
+ | GTGGCGAGAA | ||
+ | AGGAAGGGAA | ||
+ | 361 | ||
+ | GAAAGCGAAA | ||
+ | GGAGCGGGCG | ||
+ | CTAGGGCGCT | ||
+ | GGCAAGTGTA | ||
+ | GCGGTCACGC | ||
+ | TGCGCGTAAC | ||
+ | 421 | ||
+ | CACCACACCC | ||
+ | GCCGCGCTTA | ||
+ | ATGCGCCGCT | ||
+ | ACAGGGCGCG | ||
+ | TCCCATTCGC | ||
+ | CATTCAGGCT | ||
+ | 481 | ||
+ | GCGCAACTGT | ||
+ | TGGGAAGGGC | ||
+ | GATCGGTGCG | ||
+ | GGCCTCTTCG | ||
+ | CTATTACGCC | ||
+ | AGCTGGCGAA | ||
+ | 541 | ||
+ | AGGGGGATGT | ||
+ | GCTGCAAGGC | ||
+ | GATTAAGTTG | ||
+ | GGTAACGCCA | ||
+ | GGGTTTTCCC | ||
+ | AGTCACGACG | ||
+ | 601 | ||
+ | TTGTAAAACG | ||
+ | ACGGCCAGTG | ||
+ | AATTGTAATA | ||
+ | CGACTCACTA | ||
+ | TAGGGCGAAT | ||
+ | TGGGTACCGG | ||
+ | 661 | ||
+ | GCCCCCCCTC | ||
+ | GAGGTCGACG | ||
+ | GTATCGATAA | ||
+ | GCTTGATATC | ||
+ | GAATTCCTGC | ||
+ | AGCCCGGGGG | ||
+ | 721 | ||
+ | ATCCACTAGT | ||
+ | TCTAGAGCGG | ||
+ | CCGCCACCGC | ||
+ | GGTGGAGCTC | ||
+ | CAGCTTTTGT | ||
+ | TCCCTTTAGT | ||
+ | 781 | ||
+ | GAGGGTTAAT | ||
+ | TTCGAGCTTG | ||
+ | GCGTAATCAT | ||
+ | GGTCATAGCT | ||
+ | GTTTCCTGTG | ||
+ | TGAAATTGTT | ||
+ | 841 | ||
+ | ATCCGCTCAC | ||
+ | AATTCCACAC | ||
+ | AACATACGAG | ||
+ | CCGGAAGCAT | ||
+ | AAAGTGTAAA | ||
+ | GCCTGGGGTG | ||
+ | 901 | ||
+ | CCTAATGAGT | ||
+ | GAGCTAACTC | ||
+ | ACATTAATTG | ||
+ | CGTTGCGCTC | ||
+ | ACTGCCCGCT | ||
+ | TTCCAGTCGG | ||
+ | 961 | ||
+ | GAAACCTGTC | ||
+ | GTGCCAGCTG | ||
+ | CATTAATGAA | ||
+ | TCGGCCAACG | ||
+ | CGCGGGGAGA | ||
+ | GGCGGTTTGC | ||
+ | 1021 | ||
+ | GTATTGGGCG | ||
+ | CTCTTCCGCT | ||
+ | TCCTCGCTCA | ||
+ | CTGACTCGCT | ||
+ | GCGCTCGGTC | ||
+ | GTTCGGCTGC | ||
+ | 1081 | ||
+ | GGCGAGCGGT | ||
+ | ATCAGCTCAC | ||
+ | TCAAAGGCGG | ||
+ | TAATACGGTT | ||
+ | ATCCACAGAA | ||
+ | TCAGGGGATA | ||
+ | 1141 | ||
+ | ACGCAGGAAA | ||
+ | GAACATGTGA | ||
+ | GCAAAAGGCC | ||
+ | AGCAAAAGGC | ||
+ | CAGGAACCGT | ||
+ | AAAAAGGCCG | ||
+ | 1201 | ||
+ | CGTTGCTGGC | ||
+ | GTTTTTCCAT | ||
+ | AGGCTCCGCC | ||
+ | CCCCTGACGA | ||
+ | GCATCACAAA | ||
+ | AATCGACGCT | ||
+ | 1261 | ||
+ | CAAGTCAGAG | ||
+ | GTGGCGAAAC | ||
+ | CCGACAGGAC | ||
+ | TATAAAGATA | ||
+ | CCAGGCGTTT | ||
+ | CCCCCTGGAA | ||
+ | 1321 | ||
+ | GCTCCCTCGT | ||
+ | GCGCTCTCCT | ||
+ | GTTCCGACCC | ||
+ | TGCCGCTTAC | ||
+ | CGGATACCTG | ||
+ | TCCGCCTTTC | ||
+ | 1381 | ||
+ | TCCCTTCGGG | ||
+ | AAGCGTGGCG | ||
+ | CTTTCTCATA | ||
+ | GCTCACGCTG | ||
+ | TAGGTATCTC | ||
+ | AGTTCGGTGT | ||
+ | 1441 | ||
+ | AGGTCGTTCG | ||
+ | CTCCAAGCTG | ||
+ | GGCTGTGTGC | ||
+ | ACGAACCCCC | ||
+ | CGTTCAGCCC | ||
+ | GACCGCTGCG | ||
+ | 1501 | ||
+ | CCTTATCCGG | ||
+ | TAACTATCGT | ||
+ | CTTGAGTCCA | ||
+ | ACCCGGTAAG | ||
+ | ACACGACTTA | ||
+ | TCGCCACTGG | ||
+ | 1561 | ||
+ | CAGCAGCCAC | ||
+ | TGGTAACAGG | ||
+ | ATTAGCAGAG | ||
+ | CGAGGTATGT | ||
+ | AGGCGGTGCT | ||
+ | ACAGAGTTCT | ||
+ | 1621 | ||
+ | TGAAGTGGTG | ||
+ | GCCTAACTAC | ||
+ | GGCTACACTA | ||
+ | GAAGGACAGT | ||
+ | ATTTGGTATC | ||
+ | TGCGCTCTGC | ||
+ | 1681 | ||
+ | TGAAGCCAGT | ||
+ | TACCTTCGGA | ||
+ | AAAAGAGTTG | ||
+ | GTAGCTCTTG | ||
+ | ATCCGGCAAA | ||
+ | CAAACCACCG | ||
+ | 1741 | ||
+ | CTGGTAGCGG | ||
+ | TGGTTTTTTT | ||
+ | GTTTGCAAGC | ||
+ | AGCAGATTAC | ||
+ | GCGCAGAAAA | ||
+ | AAAGGATCTC | ||
+ | 1801 | ||
+ | AAGAAGATCC | ||
+ | TTTGATCTTT | ||
+ | TCTACGGGGT | ||
+ | CTGACGCTCA | ||
+ | GTGGAACGAA | ||
+ | AACTCACGTT | ||
+ | 1861 | ||
+ | AAGGGATTTT | ||
+ | GGTCATGAGA | ||
+ | TTATCAAAAA | ||
+ | GGATCTTCAC | ||
+ | CTAGATCCTT | ||
+ | TTAAATTAAA | ||
+ | 1921 | ||
+ | AATGAAGTTT | ||
+ | TAAATCAATC | ||
+ | TAAAGTATAT | ||
+ | ATGAProxy-Connection: keep-alive | ||
+ | Cache-Control: max-age=0 | ||
+ | |||
+ | AAAC | ||
+ | TTGGTCTGAC | ||
+ | AGTTACCAAT | ||
+ | 1981 | ||
+ | GCTTAATCAG | ||
+ | TGAGGCACCT | ||
+ | ATCTCAGCGA | ||
+ | TCTGTCTATT | ||
+ | TCGTTCATCC | ||
+ | ATAGTTGCCT | ||
+ | 2041 | ||
+ | GACTCCCCGT | ||
+ | CGTGTAGATA | ||
+ | ACTACGATAC | ||
+ | GGGAGGGCTT | ||
+ | ACCATCTGGC | ||
+ | CCCAGTGCTG | ||
+ | 2101 | ||
+ | CAATGATACC | ||
+ | GCGAGACCCA | ||
+ | CGCTCACCGG | ||
+ | CTCCAGATTT | ||
+ | ATCAGCAATA | ||
+ | AACCAGCCAG | ||
+ | 2161 | ||
+ | CCGGAAGGGC | ||
+ | CGAGCGCAGA | ||
+ | AGTGGTCCTG | ||
+ | CAACTTTATC | ||
+ | CGCCTCCATC | ||
+ | CAGTCTATTA | ||
+ | 2221 | ||
+ | ATTGTTGCCG | ||
+ | GGAAGCTAGA | ||
+ | GTAAGTAGTT | ||
+ | CGCCAGTTAA | ||
+ | TAGTTTGCGC | ||
+ | AACGTTGTTG | ||
+ | 2281 | ||
+ | CCATTGCTAC | ||
+ | AGGCATCGTG | ||
+ | GTGTCACGCT | ||
+ | CGTCGTTTGG | ||
+ | TATGGCTTCA | ||
+ | TTCAGCTCCG | ||
+ | 2341 | ||
+ | GTTCCCAACG | ||
+ | ATCAAGGCGA | ||
+ | GTTACATGAT | ||
+ | CCCCCATGTT | ||
+ | GTGCAAAAAA | ||
+ | GCGGTTAGCT | ||
+ | 2401 | ||
+ | CCTTCGGTCC | ||
+ | TCCGATCGTT | ||
+ | GTCAGAAGTA | ||
+ | AGTTGGCCGC | ||
+ | AGTGTTATCA | ||
+ | CTCATGGTTA | ||
+ | 2461 | ||
+ | TGGCAGCACT | ||
+ | GCATAATTCT | ||
+ | CTTACTGTCA | ||
+ | TGCCATCCGT | ||
+ | AAGATGCTTT | ||
+ | TCTGTGACTG | ||
+ | 2521 | ||
+ | GTGAGTACTC | ||
+ | AACCAAGTCA | ||
+ | TTCTGAGAAT | ||
+ | AGTGTATGCG | ||
+ | GCGACCGAGT | ||
+ | TGCTCTTGCC | ||
+ | 2581 | ||
+ | CGGCGTCAAT | ||
+ | ACGGGATAAT | ||
+ | ACCGCGCCAC | ||
+ | ATAGCAGAAC | ||
+ | TTTAAAAGTG | ||
+ | CTCATCATTG | ||
+ | 2641 | ||
+ | GAAAACGTTC | ||
+ | TTCGGGGCGA | ||
+ | AAACTCTCAA | ||
+ | GGATCTTACC | ||
+ | GCTGTTGAGA | ||
+ | TCCAGTTCGA | ||
+ | 2701 | ||
+ | TGTAACCCAC | ||
+ | TCGTGCACCC | ||
+ | AACTGATCTT | ||
+ | CAGCATCTTT | ||
+ | TACTTTCACC | ||
+ | AGCGTTTCTG | ||
+ | 2761 | ||
+ | GGTGAGCAAA | ||
+ | AACAGGAAGG | ||
+ | CAAAATGCCG | ||
+ | CAAAAAAGGG | ||
+ | AATAAGGGCG | ||
+ | ACACGGAAAT | ||
+ | 2821 | ||
+ | GTTGAATACT | ||
+ | CATACTCTTC | ||
+ | CTTTTTCAAT | ||
+ | ATTATTGAAG | ||
+ | CATTTATCAG | ||
+ | GGTTATTGTC |
Latest revision as of 19:14, 29 July 2008
pBluescript SK
[http://www.addgene.org/pgvec1?f=v&cmd=showvecinfo&vectorid=5495 Plasmid map source information]
Sequence
1 CACCTAAATT GTAAGCGTTA ATATTTTGTT AAAATTCGCG TTAAATTTTT GTTAAATCAG 61 CTCATTTTTT AACCAATAGG CCGAAATCGG CAAAATCCCT TATAAATCAA AAGAATAGAC 121 CGAGATAGGG TTGAGTGTTG TTCCAGTTTG GAACAAGAGT CCACTATTAA AGAACGTGGA 181 CTCCAACGTC AAAGGGCGAA AAACCGTCTA TCAGGGCGAT GGCCCACTAC GTGAACCATC 241 ACCCTAATCA AGTTTTTTGG GGTCGAGGTG CCGTAAAGCA CTAAATCGGA ACCCTAAAGG 301 GAGCCCCCGA TTTAGAGCTT GACGGGGAAA GCCGGCGAAC GTGGCGAGAA AGGAAGGGAA 361 GAAAGCGAAA GGAGCGGGCG CTAGGGCGCT GGCAAGTGTA GCGGTCACGC TGCGCGTAAC 421 CACCACACCC GCCGCGCTTA ATGCGCCGCT ACAGGGCGCG TCCCATTCGC CATTCAGGCT 481 GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC AGCTGGCGAA 541 AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC AGTCACGACG 601 TTGTAAAACG ACGGCCAGTG AATTGTAATA CGACTCACTA TAGGGCGAAT TGGGTACCGG 661 GCCCCCCCTC GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCCTGC AGCCCGGGGG 721 ATCCACTAGT TCTAGAGCGG CCGCCACCGC GGTGGAGCTC CAGCTTTTGT TCCCTTTAGT 781 GAGGGTTAAT TTCGAGCTTG GCGTAATCAT GGTCATAGCT GTTTCCTGTG TGAAATTGTT 841 ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG 901 CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG 961 GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC 1021 GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC 1081 GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA 1141 ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG 1201 CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT 1261 CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA 1321 GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC 1381 TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT 1441 AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG 1501 CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG 1561 CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT 1621 TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC TGCGCTCTGC 1681 TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG 1741 CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC 1801 AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT 1861 AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA 1921 AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAProxy-Connection: keep-alive Cache-Control: max-age=0
AAAC TTGGTCTGAC AGTTACCAAT 1981 GCTTAATCAG TGAGGCACCT ATCTCAGCGA TCTGTCTATT TCGTTCATCC ATAGTTGCCT 2041 GACTCCCCGT CGTGTAGATA ACTACGATAC GGGAGGGCTT ACCATCTGGC CCCAGTGCTG 2101 CAATGATACC GCGAGACCCA CGCTCACCGG CTCCAGATTT ATCAGCAATA AACCAGCCAG 2161 CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG CAACTTTATC CGCCTCCATC CAGTCTATTA 2221 ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT CGCCAGTTAA TAGTTTGCGC AACGTTGTTG 2281 CCATTGCTAC AGGCATCGTG GTGTCACGCT CGTCGTTTGG TATGGCTTCA TTCAGCTCCG 2341 GTTCCCAACG ATCAAGGCGA GTTACATGAT CCCCCATGTT GTGCAAAAAA GCGGTTAGCT 2401 CCTTCGGTCC TCCGATCGTT GTCAGAAGTA AGTTGGCCGC AGTGTTATCA CTCATGGTTA 2461 TGGCAGCACT GCATAATTCT CTTACTGTCA TGCCATCCGT AAGATGCTTT TCTGTGACTG 2521 GTGAGTACTC AACCAAGTCA TTCTGAGAAT AGTGTATGCG GCGACCGAGT TGCTCTTGCC 2581 CGGCGTCAAT ACGGGATAAT ACCGCGCCAC ATAGCAGAAC TTTAAAAGTG CTCATCATTG 2641 GAAAACGTTC TTCGGGGCGA AAACTCTCAA GGATCTTACC GCTGTTGAGA TCCAGTTCGA 2701 TGTAACCCAC TCGTGCACCC AACTGATCTT CAGCATCTTT TACTTTCACC AGCGTTTCTG 2761 GGTGAGCAAA AACAGGAAGG CAAAATGCCG CAAAAAAGGG AATAAGGGCG ACACGGAAAT 2821 GTTGAATACT CATACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC