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Team:Hawaii/Notebook/2008-07-30
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===[[Team:Hawaii/PCR Amplification of pRL1383a|PCR amplification of pRL1383a]]=== | ===[[Team:Hawaii/PCR Amplification of pRL1383a|PCR amplification of pRL1383a]]=== | ||
| - | [[Image:gel_PCR_7_30_08_oriV_aada_rep.jpg|right|thumb| | + | [[Image:gel_PCR_7_30_08_oriV_aada_rep.jpg|right|thumb|150px|PCR amplification of oriV, aada, rep.]] |
:<strong> Margaret </strong> | :<strong> Margaret </strong> | ||
Revision as of 23:00, 30 July 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Gel Purification
- Grace
- Purified B0030 and B0024 from gel
- B0030 = 21.9 ng/μl
- B0024 = 5.7 ng/μl
Restriction Digest
- Grace
- Digested 5 μl GFP, GFPf, and nir PCR products with EcoRI and SpeI
- Sequentially digested 10 μl pRL1383a and 5 μl J33207 PCR product with BamHI and HindIII
- Used Roche enzymes and Roche buffer M (buffer B also works) from SC.
- Ran digests on gel
PCR amplification of pRL1383a
- Margaret
- ran a gel from yesterday's PCR amplification of oriV, aadA, and rep regions.
Mini-Prep
- Margaret
- Plasmid Prep of [http://partsregistry.org/Part:BBa_I51020 BBa_I51020],[http://partsregistry.org/Part:pSB1A7 pSB1A7], [http://partsregistry.org/Part:BBa_B0015 BBa_B0015]
Colony PCR
- Margaret
- verify the insert of the oriT construct and [http://partsregistry.org/Part:BBa_I14032 BBa_I14032]
Drylab Work
Name of Task
- name of person/people who performed the task
- Summary of task and what was done. Link to experiment for detailed notes if necessary.
- e.g. read through papers, worked on proposal, etc.
Discussion
The gel purification kit is almost out of PE. If we need more before our order comes in, there is PE in the Qiaprep Spin Miniprep kit (just remember to add EtOH before use).
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
