Wisconsin: Lignin Project/1 July 2008

From 2008.igem.org

(Difference between revisions)
(New page: == Team Sorbitol == The overnight PCR reaction failed. Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before. The digestion was veri...)
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== Team Sorbitol ==
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<div style="background: #000; padding: 10px;">
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The overnight PCR reaction failed.
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<div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div>
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Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before.
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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
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The digestion was verified on a gel and extracted using the Qiagen kit.
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'''Team Sorbitol:'''<br>
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The overnight PCR reaction failed.<br>
-
This time we obtained enough insert to do a ligation but not enough vector.
+
Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before.<br>
-
 
+
The digestion was verified on a gel and extracted using the Qiagen kit.<br>
-
To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C
+
This time we obtained enough insert to do a ligation but not enough vector.<br>
 +
To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C<br>

Revision as of 15:29, 8 August 2008

Igemwibanner.gif
Team Sorbitol:
The overnight PCR reaction failed.
Used the purified PCR product from yesterday to set up a digestion (along with pBAD30) as described before.
The digestion was verified on a gel and extracted using the Qiagen kit.
This time we obtained enough insert to do a ligation but not enough vector.
To solve this we grew 40mL of DH5a with pBAD30 overnight at 30C
Retrieved from "http://2008.igem.org/Wisconsin:_Lignin_Project/1_July_2008"