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Wisconsin: Lignin Project/2 July 2008
From 2008.igem.org
(Difference between revisions)
(New page: == Team Sorbitol == The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before. We were able to extract enough plasmid DNA to perform a ligation. We then set ...) |
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We then set up a ligation with the following rations of vector to insert: | We then set up a ligation with the following rations of vector to insert: | ||
V:I | V:I | ||
| + | |||
3 1 | 3 1 | ||
| + | |||
1 1 | 1 1 | ||
| + | |||
1 3 | 1 3 | ||
| + | |||
1 5 | 1 5 | ||
| + | |||
1 0 | 1 0 | ||
| + | |||
0 1 | 0 1 | ||
alowed to ligate at 4C overnight. | alowed to ligate at 4C overnight. | ||
Revision as of 20:16, 31 July 2008
Team Sorbitol
The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before.
We were able to extract enough plasmid DNA to perform a ligation.
We then set up a ligation with the following rations of vector to insert: V:I
3 1
1 1
1 3
1 5
1 0
0 1
alowed to ligate at 4C overnight.
