Wisconsin: Lignin Project/2 July 2008

From 2008.igem.org

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== Team Sorbitol ==
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<div style="background: #000; padding: 10px;">
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The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before.
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<div style="width:800px; height:147px; border:0px; margin:0px;">[[Image:Igemwibanner.gif]]</div>
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We were able to extract enough plasmid DNA to perform a ligation.
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{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
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We then set up a ligation with the following rations of vector to insert:
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|'''Team Sorbitol:'''<br>
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The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before.<br>
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V:I
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We were able to extract enough plasmid DNA to perform a ligation.<br>
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We then set up a ligation with the following rations of vector to insert: 5:1, 3:1, 1:1, 1:3, 1:5, 1:0, 0:1<br>
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3 1
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The mixture wasallowed to ligate at 4C overnight.<br>
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|}
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1 1
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1 3
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1 5
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1 0
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0 1
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alowed to ligate at 4C overnight.
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Revision as of 15:32, 8 August 2008

Igemwibanner.gif
Team Sorbitol:

The pBAD30 was harvested using a Qiagen Miniprep kit and digested as described before.
We were able to extract enough plasmid DNA to perform a ligation.
We then set up a ligation with the following rations of vector to insert: 5:1, 3:1, 1:1, 1:3, 1:5, 1:0, 0:1
The mixture wasallowed to ligate at 4C overnight.

Retrieved from "http://2008.igem.org/Wisconsin:_Lignin_Project/2_July_2008"