Team:Valencia/Notebook/July

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Revision as of 08:48, 4 August 2008

July 1st

- We prepared some glycerol stock from the four Saccharomyces cerevisiae strains we received from Eduardo Rial.

- We calibrated our calorimeters with hot water.

July 2nd

- We prepared our first SD medium.

- We calibrated our calorimeters with hot water.

July 4th

- We prepared some plates for inoculum.

July 7th

- We prepared some inoculum.

July 8th

- We carried out the pilot experiment.

July 9th

- We characterized culture growth in our calorimeters.

july 10th

- Trying to see if there is basal rate of UCP1...

july 11th

- It looks like our Saccharomyces cerevisiae strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure.

july 12th

- We obtained the same results.

july 14th

- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.

july 15th

- We prepared inoculum.

july 16th

- Trying to find out which is the best moment for activating UCP1...

july 17th

- Our lab electricity went of during the night. How many experiments will have ruined?

july 18th

- we carried out the pilot experiment. We see weird oscillations.

july 20th

- we stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.

july 21st

- We carried out the pilot experiment again. Weird results, we need to repeat it.

july 22nd

- We repaired our calorimeters. They were the reason for yesterday weird results.

july 23rd

- We repeated the pilot experiment.

july 24th

- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste...

july 25th

- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.

july 26th

- We obtain good results.

july 28th

microcalorimeter isn't OK, we try to repair and now.....calibrate once again!!!!

july 29th

why Saccharomyces cerevisiae don't want growth???? the temperature its OK like the medium.....PLEASE WAIT.......

@@ Line 125: / Line 125: @@
 - We prepared some plates for inoculum.
-=== july 7th (san fermin)===
+=== July 7th ===
--	inoculum
+- We prepared some inoculum. <!-- prepared or inoculate?-->
-=== july 8th ===
+=== July 8th ===
--pilot experiment
+- We carried out the pilot experiment.
+===July 9th===
+- We characterized culture growth in our calorimeters. <!-- just one strain?-->
-===july 9th===
-characteize growth of ''Saccharomyces cerevisiae''
 ===july 10th===
-there are a basal rate of UCP1??? we go to check it!!!!
+- Trying to see if there is basal rate of UCP1...
 ===july 11th===
-yes!!!! our ''Saccharomyces cerevisiae'' have a basal rate of UCP1.....but its real or it's a dream???
+- It looks like our ''Saccharomyces cerevisiae'' strains have a basal rate of UCP1... We'll have to repeat the experiment to be sure.
 ===july 12th===
-yes yes yes!!!! the same results as yesterday!!! WE'RE HAPPY!!!
+- We obtained the same results.
 ===july 14th===
-the plate is dry.....the water evaporeted.....WHY???? the first mystery of valencia iGEM 2008
+- The plate is completely dry,the water evaporeted...WHY???? the first unsolved mystery of valencia iGEM 2008.
 ===july 15th===
-- inoculum
+- We prepared inoculum.
 ===july 16th===
-which is the best moment for activation UCP1???? we go to find out it!!!
+- Trying to find out which is the best moment for activating UCP1...
 ===july 17th===
-where's the laboratory light????? we don't know....... bye bye experiment!!!!!!
+- Our lab electricity went of during the night. How many experiments will have ruined?
 ===july 18th===
-- ''Saccharomyces cerevisiae'' needs glucose to survive???? we check it.....''Saccharomyces cerevisiae'' needs glucose to growth.
+<!-- - ''Saccharomyces cerevisiae'' needs glucose to survive???? we check it.....''Saccharomyces cerevisiae'' needs glucose to growth.-->
-- pilot experiment
+- we carried out the pilot experiment. We see weird oscillations.
 ===july 20th===
-we stop the shake and see the results of pilot experiment with no shaking.
+- we stopped the shaking and carried out the experiment again to see whether the oscillations were related to the shaking.
 ===july 21st===
-another pilot experiment but....this results???? this not ok.......we should repeat this.....
+- We carried out the pilot experiment again. Weird results, we need to repeat it.
 ===july 22nd===
-we repair "microcalorimeter"
+- We repaired our calorimeters. They were the reason for yesterday weird results.
 ===july 23rd===
-repeat pilot experiment (once again)
+- We repeated the pilot experiment.
 ===july 24th===
-"IDEAS DE BOMBERO" this is a spanish set phrase,it means: this is something stupid but.....
+- Our male group members decided to carried out their own particular experiment involving parafilm, plasticine and teeth filling paste...
 ===july 25th===
-other "IDEA DE BOMBERO": one thermocouple is cover with plasticine and the others no but...
+- Another curious experiment was carried out: one thermocouple was covered with plasticine while the others weren't.
 ===july 26th===
-...the results it's OK!!!!
+- We obtain good results.
 ===july 28th===