Team:Valencia/Modeling

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Revision as of 18:50, 7 August 2008

Oxidative phosphorylation coupled with thermogenine expression model

Our project tries to implement a controlled heating system inside Saccharomyces cerevisiae. The main variables to be taken into account are temperature production and ATP flow. We need to formulate an effective model for the oxidative phosphorylation that couples the genetic expression of the thermogenine with the efficiency of the respiratory chain in ATP production. It will allow us to evaluate the effect on the energy flow produced by the thermogenic activity.

Notwithstanding the lack of a specific respiratory chain model in yeast, we are trying to develop our differential equations based on kinetic data collected from the literature. We will later compare our model with other sets of differential equations thought to describe the behavior of skeletal muscle mitochondrion.

References

Mitochondrial Respiration and ATP Production Are Significantly Impaired in Striatal Cells Expressing Mutant Huntingtin*

Tamara Milakovic and Gail V. W. Johnson

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-==Calculating temperature range==
+==Oxidative phosphorylation coupled with thermogenine expression model==
-We try to establish the range of temperatures within we expect that thermogenine will keep the culture at. The minimum value of temperature is, evidently, the corresponding to the culture conditions. The maximal quote was determined by supposing that the proton motrive force was used solely in heat production mediated by thermogenine. The following calculations were made taking into account this assumption.
-We used the published rate (Milakovik et al 2005) of ATP produced per second per mitochondria to calculate the energy produce per proton dissipated through the thermogenine  per second per mitochondria.
+Our project tries to implement a controlled heating system inside ''Saccharomyces cerevisiae''. The main variables to be taken into account are temperature production and ATP flow. We need to formulate an effective model for the oxidative  phosphorylation that couples the genetic expression of the thermogenine with the efficiency of the respiratory chain in ATP production. It will allow us to evaluate the effect on the energy flow produced by the thermogenic activity.
-.5 nmol/min = 2.92 *10-10 mol/s = 1.76*1014 ATP particles/s
-If ATP synthase needs four protons to produce one molecule of ATP  then we can calculate easily the proton flow through complex V.
-.03*10^14 p+/s
-The free energy associated to a single proton pass through the ATP synthase is 20 Kj/mol, just by multiplying.
-.32* 1023 Kj/p+
-Thus the energy flow is calculated by multiplying both figures and adjusting the result to the proper units:
-.33*10-5 J/s
-Knowing the number of mitochondria per cell, the optical density of the culture and the amount of medium, we can effortlessly establish the maximum temperature with the help of the figure calculated above.
+Notwithstanding the lack of a specific respiratory chain model in yeast, we are trying to develop our differential equations based on kinetic data collected from the literature. We will later compare our model with other sets of differential equations thought to describe the behavior of skeletal muscle mitochondrion.
 ==References==