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Team:Hawaii/Notebook/2008-08- 7
From 2008.igem.org
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===Verification of transformants=== | ===Verification of transformants=== | ||
| - | [[Image:080708colonyPCR.jpg|right|thumb| | + | [[Image:080708colonyPCR.jpg|right|thumb|500px|EtBr stained 2.5% agarose gel ran at 95V for ---hours. Ten microliters of the PCR reaction were loaded into each well.]] |
:<strong>Grace</strong> | :<strong>Grace</strong> | ||
Revision as of 10:01, 8 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Plasmid prep
- Krystle
- Finished plasmid prep of BB-pRL1383a, nir, GFPf from yesterday
Cyanobacteria seed stocks
- Krystle
- Reinoculated liquid and solid media seed stocks
Verification of transformants
- Grace
| Construct | Colony forming units |
|---|---|
| nir + B0030 (rbs) | 2 + 1 cluster of colonies |
| plac (I14032) + B0030 (rbs) | 37 |
| GFP (E0040) + B0015 (tt) | 8 |
| GFPf + B0015 (tt) | 4 |
| lac device (J33207) + B0015 (tt) | 56 |
- Colony PCR of transformants
- Ran on EtBr stained 2.5% agarose gel at 95V for --- hours.
Discussion
Quote of the Day
Gernot: "So would you eat the E. coli to save them if we lose the -80C in like a war?"
Loren: "Well, I'll eat them, but you'll be collecting them."
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
