"
Team:Hawaii/Notebook/2008-08- 8
From 2008.igem.org
(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Restreaked nir+rbs and I14032+rbs constructs=== :<strong> Grace</strong> ===Prep for sequencing=== [[Image:080808PCR.j...) |
(→Prep for sequencing) |
||
| Line 14: | Line 14: | ||
::* Colony PCR indicates no ligation. Picked new colony, sequence to confirm failure. | ::* Colony PCR indicates no ligation. Picked new colony, sequence to confirm failure. | ||
:* Gel purified all PCR rxns (we still have a problem with contaminant DNA/shadow bands) and desired bands were extracted from gel | :* Gel purified all PCR rxns (we still have a problem with contaminant DNA/shadow bands) and desired bands were extracted from gel | ||
| + | ::* 2% agarose gel ran at 60v for 2 hours | ||
:* Determined DNA concentrations via nanodrop spectrometer | :* Determined DNA concentrations via nanodrop spectrometer | ||
:* Prepared samples and sent to CORE Hawaii for sequencing | :* Prepared samples and sent to CORE Hawaii for sequencing | ||
| - | |||
= Discussion = | = Discussion = | ||
Revision as of 18:37, 8 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Restreaked nir+rbs and I14032+rbs constructs
- Grace
Prep for sequencing
- Grace
- 25 μl PCR reactions of nir+rbs, I14032+rbs, slr1, slr2, BB-pRL1383a
- Colony PCRs seem to indicate success
- 25 μl PCR reactions of GFP+tt, GFPf+tt, J33207+tt
- Colony PCR indicates no ligation. Picked new colony, sequence to confirm failure.
- Gel purified all PCR rxns (we still have a problem with contaminant DNA/shadow bands) and desired bands were extracted from gel
- 2% agarose gel ran at 60v for 2 hours
- Determined DNA concentrations via nanodrop spectrometer
- Prepared samples and sent to CORE Hawaii for sequencing
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
