Wisconsin:Lignin Project/7 August 2008

From 2008.igem.org

(Difference between revisions)
Line 4: Line 4:
{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
{|align="justify" style="color:#aada84;background-color:#000;" width="800 px"
-
|'''Team Fungus:'''<br>
+
|'''Team Sorbitol:'''<br>
 +
Set up a Digestion of the srlD PCR product as well as the pET28a vector.  The Products were verified on a gel and extracted using a gel purification kit.
 +
 
 +
'''Team Fungus:'''<br>
Transformed into Rosetta-gami 2(DE3)pLysS.<br>
Transformed into Rosetta-gami 2(DE3)pLysS.<br>
Made overnight cultures of colonies 5 & 6 in LB + KAN (30 ug/mL) media. <br>
Made overnight cultures of colonies 5 & 6 in LB + KAN (30 ug/mL) media. <br>
|}
|}

Revision as of 16:46, 28 October 2008

Igemwibanner.gif
Team Sorbitol:

Set up a Digestion of the srlD PCR product as well as the pET28a vector. The Products were verified on a gel and extracted using a gel purification kit.

Team Fungus:
Transformed into Rosetta-gami 2(DE3)pLysS.
Made overnight cultures of colonies 5 & 6 in LB + KAN (30 ug/mL) media.