Alberta NINT/27 May 2008

From 2008.igem.org

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lab work (SD): DNA digest of K102000 with SpeI + PstI.  Incubated at 37 C for 1 hour.  Run on 2% agarose gel.  ~3 kbp fragment expected.  Ligated K102000 with E0433 and K102000 with null_output.
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lab work (SD):  
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lab work (JD): Recovered Part BBA_R011 from iGEM package, transformed XL1-B cells and plated on LB+Amp100 plates.  Created 2 O/Ns as well.
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            DNA digest of K102000 with SpeI + PstI.  Incubated at 37 C for 1 hour. 
 +
            Run on 2% agarose gel.  ~3 kbp fragment expected. 
 +
            Ligated K102000 with E0433 and K102000 with null_output.
 +
 
 +
lab work (JD):  
 +
 
 +
            Recovered Part BBA_R011 from iGEM package  
 +
            Transformed XL1-B cells and plated on LB+Amp100 plates.   
 +
            Created 2 O/Ns as well.

Revision as of 21:43, 6 June 2008

lab work (SD): 

           DNA digest of K102000 with SpeI + PstI.  Incubated at 37 C for 1 hour.  
           Run on 2% agarose gel.  ~3 kbp fragment expected.  
           Ligated K102000 with E0433 and K102000 with null_output.

lab work (JD): 

            Recovered Part BBA_R011 from iGEM package 
            Transformed XL1-B cells and plated on LB+Amp100 plates.  
            Created 2 O/Ns as well.
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