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Alberta NINT/29 May 2008
From 2008.igem.org
(Difference between revisions)
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| - | lab work (SD): | + | lab work (SD): |
| - | lab work (JD): Gel purified R0011 band from gel electrophoresis. Carried out quantitative analysis | + | Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09). |
| + | DNA was run on 2% agarose gel. All 9 samples were identical on the gel. | ||
| + | K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were selected at random | ||
| + | inoculated into LB + amp50 culture tubes and incubated overnight at 37 C. | ||
| + | |||
| + | lab work (JD): | ||
| + | |||
| + | Gel purified R0011 band from gel electrophoresis. | ||
| + | Carried out quantitative analysis | ||
| + | Carried out ligation to create K102001 (R0011+T/A1) | ||
| + | Transformed XL1-B cells with K102001. | ||
| + | Prepared R0011 for sequencing and dropped it off. | ||
Revision as of 21:46, 6 June 2008
lab work (SD):
Colony PCR of 9 colonies from K102002 (XL1-B) and 9 colonies from K102004 (XL1-B) (plates from 28/05/09).
DNA was run on 2% agarose gel. All 9 samples were identical on the gel.
K102002.2, K102002.4, K102002.5, K102002.8, K102004.1, K102004.3, K102004.4, and K102004.5 were selected at random
inoculated into LB + amp50 culture tubes and incubated overnight at 37 C.
lab work (JD):
Gel purified R0011 band from gel electrophoresis.
Carried out quantitative analysis
Carried out ligation to create K102001 (R0011+T/A1)
Transformed XL1-B cells with K102001.
Prepared R0011 for sequencing and dropped it off.
