Team:Hawaii/Notebook/2008-08-15

From 2008.igem.org

(Difference between revisions)
(→Reconstruction of BB-pRL1383a)
(→Quote of the Day)
Line 27: Line 27:
= Quote of the Day =
= Quote of the Day =
-
<blockquote>''History is the only laboratory we have in which to test the consequences of thought.'' - Étienne Gilson</blockquote>
+
<blockquote>''I'm waiting for the terminator to come in...'' - Krystle</blockquote>
{{Team:Hawaii/Footer}}
{{Team:Hawaii/Footer}}
__NOTOC__
__NOTOC__

Revision as of 02:06, 16 August 2008

Projects Events Resources
Sponsors Experiments Milestones Protocols
Notebook (t) Meetings (t)

Things we did today

Wetlab work

Reconstruction of BB-pRL1383a

EtBr stained 1% agarose gel ran at 72V for ... Thirty-five microliters of RE digested BB-pRL1383a and fifty microliters of RE digested J33207 were loaded.
Grace
  • PCR amplification of J33207
  • ExoSAP'd PCR product
  • RE digested of J33207 and BB-pRL1383a (w/ GFP) with EcoRI and PstI
  • Ran digests on a 1% agarose gel at 72V for 1.5 hours.
  • Ladder did not run well. Bands not resolving well.
  • Redid PCR and RE digests with XbaI and PstI

Made 20X X-gal stock solution (20 mg/ml)

Grace

Drylab Work

Editing team website

Grace

Discussion

  • DH5α does not carry lacIq; therefore, IPTG is not neccessary to induce the lac promoter.
  • IPTG is necessary for induction in DB3.1 cells.

Quote of the Day

I'm waiting for the terminator to come in... - Krystle


[http://manoa.hawaii.edu/ Sponsor_UHM.gif][http://manoa.hawaii.edu/ovcrge/ Sponsor_OVCRGE.gif][http://www.ctahr.hawaii.edu Sponsor_CTAHR.gif]


Retrieved from "http://2008.igem.org/Team:Hawaii/Notebook/2008-08-15"