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Team:Hawaii/Notebook/2008-08-15
From 2008.igem.org
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== Wetlab work == | == Wetlab work == | ||
===Reconstruction of BB-pRL1383a=== | ===Reconstruction of BB-pRL1383a=== | ||
| - | [[Image:081508REdigest.jpg|right|thumb|200px|EtBr stained 1% agarose gel ran at 72V for . | + | [[Image:081508REdigest.jpg|right|thumb|200px|EtBr stained 1% agarose gel ran at 72V for 1.5 hours. Thirty-five microliters of RE digested BB-pRL1383a and fifty microliters of RE digested J33207 were loaded.]] |
:<strong> Grace</strong> | :<strong> Grace</strong> | ||
Revision as of 02:08, 16 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Reconstruction of BB-pRL1383a
- Grace
- PCR amplification of J33207
- ExoSAP'd PCR product
- RE digested of J33207 and BB-pRL1383a (w/ GFP) with EcoRI and PstI
- Ran digests on a 1% agarose gel at 72V for 1.5 hours.
- Ladder did not run well. Bands not resolving well.
- Redid PCR and RE digests with XbaI and PstI
Made 20X X-gal stock solution (20 mg/ml)
- Grace
Drylab Work
Editing team website
- Grace
Discussion
- DH5α does not carry lacIq; therefore, IPTG is not neccessary to induce the lac promoter.
- IPTG is necessary for induction in DB3.1 cells.
Quote of the Day
I'm waiting for the terminator to come in... - Krystle
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
