Team:Hawaii/Notebook/2008-08-19

From 2008.igem.org

(Difference between revisions)
(New page: {{Team:Hawaii/Header}} = Things we did today = == Wetlab work == ===Name of Task=== :<strong> name of person/people who performed the task</strong> :* Summary of task and what was done. ...)
(Wetlab work)
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= Things we did today =
= Things we did today =
== Wetlab work ==
== Wetlab work ==
-
===Name of Task===
+
===RE test===
-
:<strong> name of person/people who performed the task</strong>
+
:<strong>Krystle</strong>
-
:* Summary of task and what was done. Link to experiment for detailed notes if necessary.
+
:* Checked to see if RE are still active by cutting R0010 with EcoRI, XbaI, SpeI, and PstI individually
-
:* e.g. worked on &lt;blah experiment link&gt;, PCR, ran gel
+
:* Ran on 2% agarose gel
 +
::* R0010 was probably from a plasmid prep where cells did not lyse, i.e. no DNA in prep
 +
::* Oops, forgot to run uncut plasmid as control
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:* Set up new RE digest (w/ C0012) overnight to check enzymatic activity
 +
::* Also checked EcoRI enzyme in TKW box of lab freezer
 +
===Verification of transformants===
 +
:<strong> Grace</strong>
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[[Image:081908.jpg|right|thumb|250px|EtBr stained 2% agarose gel ran at 95V for 1 hour. Thirty microliters of RE digest and ten microliters of PCR reaction were loaded into each lane.]]
 +
:* Plasmid prep of BB-pRL1383a+J33207 plasmid to verify replacement of GFP with J33207 (lac device)
 +
:* PCR of BB-pRL1383a+J33207 plasmid preps
 +
::* Negative = water
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::* Positive = BB-pRL1383a
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:* Ran on 2% agarose gel
== Drylab Work ==
== Drylab Work ==

Revision as of 08:27, 20 August 2008

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Things we did today

Wetlab work

RE test

Krystle
  • Checked to see if RE are still active by cutting R0010 with EcoRI, XbaI, SpeI, and PstI individually
  • Ran on 2% agarose gel
  • R0010 was probably from a plasmid prep where cells did not lyse, i.e. no DNA in prep
  • Oops, forgot to run uncut plasmid as control
  • Set up new RE digest (w/ C0012) overnight to check enzymatic activity
  • Also checked EcoRI enzyme in TKW box of lab freezer

Verification of transformants

Grace
EtBr stained 2% agarose gel ran at 95V for 1 hour. Thirty microliters of RE digest and ten microliters of PCR reaction were loaded into each lane.
  • Plasmid prep of BB-pRL1383a+J33207 plasmid to verify replacement of GFP with J33207 (lac device)
  • PCR of BB-pRL1383a+J33207 plasmid preps
  • Negative = water
  • Positive = BB-pRL1383a
  • Ran on 2% agarose gel

Drylab Work

Name of Task

name of person/people who performed the task
  • Summary of task and what was done. Link to experiment for detailed notes if necessary.
  • e.g. read through papers, worked on proposal, etc.


Discussion

Quote of the Day

What?!? Grrr...... - Krystle


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