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Team:Hawaii/Notebook/2008-08-19
From 2008.igem.org
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::* Positive = BB-pRL1383a | ::* Positive = BB-pRL1383a | ||
:* Ran on 2% agarose gel | :* Ran on 2% agarose gel | ||
| + | ::* No replacement of GFP; enzymes didn't cut? | ||
== Drylab Work == | == Drylab Work == | ||
Latest revision as of 08:29, 20 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
RE test
- Krystle
- Checked to see if RE are still active by cutting R0010 with EcoRI, XbaI, SpeI, and PstI individually
- Ran on 2% agarose gel
- R0010 was probably from a plasmid prep where cells did not lyse, i.e. no DNA in prep
- Oops, forgot to run uncut plasmid as control
- Set up new RE digest (w/ C0012) overnight to check enzymatic activity
- Also checked EcoRI enzyme in TKW box of lab freezer
Verification of transformants
- Grace
- Plasmid prep of BB-pRL1383a+J33207 plasmid to verify replacement of GFP with J33207 (lac device)
- PCR of BB-pRL1383a+J33207 plasmid preps
- Negative = water
- Positive = BB-pRL1383a
- Ran on 2% agarose gel
- No replacement of GFP; enzymes didn't cut?
Drylab Work
Updated project descriptions on wiki
- Krystle
Discussion
Quote of the Day
What?!? Grrr...... - Krystle
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
