Edinburgh/25 June 2008

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Revision as of 14:01, 20 August 2008

Edinburgh iGEM 2008

Primers arrived. Made up 500μM stock solutions in EB and 10μM working solutions (f and r together) in water. Performed PCR with KOD, annealing at 55°C and extending for 38s (expected sizes are: dxs 1862 + prefix and suffix; appY 749 + prefix and suffix; glgC 1295 with no prefix or suffix (except the extra TAA). Result: all looked good, nice pure bands.
- Gel 1: markers, empty lane, dxs, appY, glgC, appY (repeat), glgC (repeat), markers.

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	* Primers arrived. Made up 500μM stock solutions in EB and 10μM working solutions (f and r together) in water. Performed PCR with KOD, annealing at 55°C and extending for 38s (expected sizes are: ''dxs'' 1862 + prefix and suffix; ''appY'' 749 + prefix and suffix; ''glgC'' 1295 with no prefix or suffix (except the extra TAA). Result: all looked good, nice pure bands.<br />		* Primers arrived. Made up 500μM stock solutions in EB and 10μM working solutions (f and r together) in water. Performed PCR with KOD, annealing at 55°C and extending for 38s (expected sizes are: ''dxs'' 1862 + prefix and suffix; ''appY'' 749 + prefix and suffix; ''glgC'' 1295 with no prefix or suffix (except the extra TAA). Result: all looked good, nice pure bands.<br />
-	'''Gel 1:''' markers, empty lane, ''dxs'', ''appY'', ''glgC'', ''appY'' (repeat), ''glgC'' (repeat), markers.	+	** '''Gel 1:''' markers, empty lane, ''dxs'', ''appY'', ''glgC'', ''appY'' (repeat), ''glgC'' (repeat), markers.