Edinburgh/12 July 2008
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- | * Result of transformation: ''crtB'' 100μl, 22 white and 10 blue; 900μl, too numerous to count, about two thirds white; a few blue showed signs of phage-like lysis. For ''crtI'' mutant, 100μl, 4 white and 2 blue; 900μl, 143 white and 25 blue. White ''crtB'' colonies were patched to '''Plate 35''', ''crtI'' to '''Plate 36'''. | + | </style> |
- | * Set up overnight cultures of possible rbs+''dxs'' clones from plate 34 for minipreps. | + | </html> |
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+ | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}} | ||
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+ | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}} | ||
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+ | |align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}} | ||
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+ | :::: '''[[Edinburgh/11_July_2008|< Previous Entry]]''' | ||
+ | |||
+ | == Week 4 == | ||
+ | === Saturday 12 July 08 === | ||
+ | |||
+ | * Result of transformation: plate 30 (pSB1A2+''crtB'' 100μl), 22 white and 10 blue; plate 31 (pSB1A2+''crtB'' 900μl), too numerous to count, about two thirds white; a few blue showed signs of phage-like lysis. For ''crtI'' mutant, plate 32 (100μl), 4 white and 2 blue; plate 33 (900μl), 143 white and 25 blue. White ''crtB'' colonies were patched to '''Plate 35''', ''crtI'' to '''Plate 36'''. | ||
+ | * Set up overnight cultures of possible rbs+''dxs'' clones from plate 34 for minipreps.<br /> | ||
+ | <br /> | ||
+ | |||
+ | :::: '''[[Edinburgh/13_July_2008|Next Entry >]]''' |
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Week 4
Saturday 12 July 08
- Result of transformation: plate 30 (pSB1A2+crtB 100μl), 22 white and 10 blue; plate 31 (pSB1A2+crtB 900μl), too numerous to count, about two thirds white; a few blue showed signs of phage-like lysis. For crtI mutant, plate 32 (100μl), 4 white and 2 blue; plate 33 (900μl), 143 white and 25 blue. White crtB colonies were patched to Plate 35, crtI to Plate 36.
- Set up overnight cultures of possible rbs+dxs clones from plate 34 for minipreps.