Edinburgh/18 August 2008

From 2008.igem.org

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=== Week 10 ===
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<div id="header">{{Template:Team:Edinburgh/Templates/notebook-entry/header}}</div>
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==== Monday 18 August 08 ====
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*M144-M147: Minipreps of cenA(1,2,3,4). M148-M151: Minipreps of cex(1,2,3,4). M152-M155: Munipreps of dxs-crtE (1,2,3,4). Another mistake made, I used methanol(920ul) instead of ethanol for M144-M147.(OG)
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:::: '''[[Edinburgh/17_August_2008|< Previous Entry]]'''
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*Minipreps 144-155 were digested with EcoRI/PstI. This need running on a gel tomorrow. (AH)  
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*X11: Maxiprep of Zea mays isoamylase 1 (isa1+pZntr22). X12: Maxiprep of Zea mays isoamylase 2 (isa2+pZntr22). (HX)  
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== Week 10 ==
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=== Monday 18 August 08 ===
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*Minipreps:
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**'''M144-M147:'''Minipreps of cenA(1,2,3,4).
 +
**'''M148-M151:''' Minipreps of cex(1,2,3,4).
 +
**'''M152-M155:''' Minipreps of dxs-crtE (1,2,3,4). Another mistake made, I used methanol(920ul) instead of ethanol for M144-M147.(OG)
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*Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH)  
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*Maxipreps:
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**'''X11:''' Zea mays isoamylase 1 (isa1+pZntr22).
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**'''X12:''' Maxiprep of Zea mays isoamylase 2 (isa2+pZntr22). (HX)  
*Combined biobricks:  
*Combined biobricks:  
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L42: dxs+lims (M137) to appY (M82).  
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**'''L42:''' dxs+lims (M137) to appY (M82).  
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L43: crtB+crtI (M109) to appY (M82).  
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**'''L43:''' crtB+crtI (M109) to appY (M82).  
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L44: pstA (M130) to lacB. (YAN)  
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**'''L44:''' pstA (M130) to lacB. (YAN)  
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*Blunt-ended ligations L45~L47 of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)  
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*Blunt-ended ligations '''L45~L47''' of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
*Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)
*Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)
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:::: '''[[Edinburgh/19_August_2008|Next Entry >]]'''

Revision as of 16:18, 6 October 2008

< Previous Entry

Week 10

Monday 18 August 08

  • Minipreps:
    • M144-M147:Minipreps of cenA(1,2,3,4).
    • M148-M151: Minipreps of cex(1,2,3,4).
    • M152-M155: Minipreps of dxs-crtE (1,2,3,4). Another mistake made, I used methanol(920ul) instead of ethanol for M144-M147.(OG)
  • Minipreps 144-155 were digested with EcoRI/PstI. These need running on a gel tomorrow. (AH)
  • Maxipreps:
    • X11: Zea mays isoamylase 1 (isa1+pZntr22).
    • X12: Maxiprep of Zea mays isoamylase 2 (isa2+pZntr22). (HX)
  • Combined biobricks:
    • L42: dxs+lims (M137) to appY (M82).
    • L43: crtB+crtI (M109) to appY (M82).
    • L44: pstA (M130) to lacB. (YAN)
  • Blunt-ended ligations L45~L47 of purified P71A (glgC mut 1+2), P71 and P72 (both glgC mut 1+2+3) to SOB2 ("son of BABEL 2"). (AM)
  • Prepared competent cells (labelled 'iGEM JM109 18/8 1-35') 1-24 = 100 microlitres each. 26-35 = 200 microlitres each. Stored at -80C. (AH, AM)
Next Entry >
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