Team:Paris/Modeling/f3bis

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[[Image:f3env.png|thumb]]
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[[Image:f3env.png|thumb]] At the steady-state, we have
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<center>[[Image:FliAeq.jpg]]</center>
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and
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<center>[[Image:EnvZeq.jpg]]</center>
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so the expression
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<center>[[Image:f3envexpr.jpg]]</center>
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gives
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 +
<center>[[Image:f3bisFliA.jpg]]</center>
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and
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<center>[[Image:f3bisEnvZ.jpg]]</center>
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and for ''calculated values of the TF'',
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<center>[[Image:f3bisFliACalc.jpg]]</center>
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and
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<center>[[Image:f3bisEnvZCalc.jpg]]</center>
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<br><br>
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{|border="1" style="text-align: center"
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|param
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|signification
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|unit
 +
|value
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|-
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|[expr(pFlhDC)]
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|expression rate of <br> pFlhDC '''with RBS E0032'''
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|nM.s<sup>-1</sup>
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|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|γ<sub>GFP</sub>
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|dilution-degradation rate <br> of GFP(mut3b)
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|s<sup>-1</sup>
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|ln(2)/3600
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|-
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|[GFP]
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|GFP concentration at steady-state
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|nM
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|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|(''fluorescence'')
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|value of the observed fluorescence
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|au
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|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|-
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|''conversion''
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|conversion ration between <br> fluorescence and concentration
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|nM.au<sup>-1</sup>
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|(1/79.429)
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|}
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<br><br>
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{|border="1" style="text-align: center"
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|param
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|signification <br> corresponding parameters in the [[Team:Paris/Modeling/Oscillations#Resulting_Equations|equations]]
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|unit
 +
|value
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|-
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|β<sub>12</sub>
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|production rate of FliA-pFlhDC '''with RBS E0032''' <br> β<sub>12</sub>
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|nM.s<sup>-1</sup>
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|
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|-
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|(K<sub>11</sub>/{coef<sub>fliA</sub>}<sup>n<sub>11</sub></sup>)
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|activation constant of FliA-pFlhDC <br> K<sub>11</sub>
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|nM<sup>n<sub>11</sub></sup>
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|
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|-
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|n<sub>11</sub>
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|complexation order of FliA-pFlhDC <br> n<sub>11</sub>
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|no dimension
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|
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|-
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|-
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|β<sub>2</sub>
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|production rate of OmpR-pFlhDC '''with RBS E0032''' <br> β<sub>2</sub>
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|nM.s<sup>-1</sup>
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|
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|-
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|(K<sub>19</sub>/{coef<sub>ompR</sub>}<sup>n<sub>19</sub></sup>)
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|activation constant of OmpR-pFlhDC <br> K<sub>19</sub>
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|nM<sup>n<sub>19</sub></sup>
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|
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|-
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|n<sub>19</sub>
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|complexation order of OmpR-pFlhDC <br> n<sub>19</sub>
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|no dimension
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|
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|}
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<br><br>
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Then, if we have time, we want to verify the expected relation
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<center>[[Image:SumFlhDC1.jpg]]</center>

Revision as of 15:30, 21 August 2008

F3env.png
At the steady-state, we have
FliAeq.jpg

and

EnvZeq.jpg

so the expression

F3envexpr.jpg

gives

F3bisFliA.jpg

and

F3bisEnvZ.jpg

and for calculated values of the TF,

F3bisFliACalc.jpg

and

F3bisEnvZCalc.jpg



param signification unit value
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032 		nM.s-1 see "findparam"
need for 20 + 20 measures
and 5x5 measures for the SUM?
γGFP dilution-degradation rate
of GFP(mut3b) 		s-1 ln(2)/3600
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the SUM?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the SUM?
conversion conversion ration between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value
β12 production rate of FliA-pFlhDC with RBS E0032
β12 		nM.s-1
(K11/{coeffliA}n11) activation constant of FliA-pFlhDC
K11 		nMn11
n11 complexation order of FliA-pFlhDC
n11 		no dimension
β2 production rate of OmpR-pFlhDC with RBS E0032
β2 		nM.s-1
(K19/{coefompR}n19) activation constant of OmpR-pFlhDC
K19 		nMn19
n19 complexation order of OmpR-pFlhDC
n19 		no dimension



Then, if we have time, we want to verify the expected relation

SumFlhDC1.jpg
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