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Team:Paris/Modeling/f3bis
From 2008.igem.org
(Difference between revisions)
| Line 63: | Line 63: | ||
|value | |value | ||
|- | |- | ||
| - | |β<sub> | + | |β<sub>12bis</sub> |
| - | |production rate of FliA-pFlhDC '''with RBS E0032''' <br> β<sub>12</sub> | + | |production rate of FliA-pFlhDC '''with RBS E0032''' <br> replace β<sub>12</sub> (not written) |
|nM.s<sup>-1</sup> | |nM.s<sup>-1</sup> | ||
| | | | ||
|- | |- | ||
| - | |(K<sub> | + | |(K<sub>11bis</sub>/{coef<sub>fliA</sub>}<sup>n<sub>11bis</sub></sup>) |
| - | |activation constant of FliA-pFlhDC <br> K<sub> | + | |activation constant of FliA-pFlhDC <br> K<sub>11bis</sub> |
| - | |nM<sup>n<sub> | + | |nM<sup>n<sub>11bis</sub></sup> |
| | | | ||
|- | |- | ||
| - | |n<sub> | + | |n<sub>11bis</sub> |
| - | |complexation order of FliA-pFlhDC <br> n<sub>11</sub> | + | |complexation order of FliA-pFlhDC <br> replace n<sub>11</sub> (not written) |
|no dimension | |no dimension | ||
| | | | ||
|- | |- | ||
|- | |- | ||
| - | |β<sub> | + | |β<sub>2bis</sub> |
| - | |production rate of | + | |production rate of EnvZ-pFlhDC '''with RBS E0032''' <br> β<sub>2bis</sub> |
|nM.s<sup>-1</sup> | |nM.s<sup>-1</sup> | ||
| | | | ||
|- | |- | ||
| - | |(K<sub> | + | |(K<sub>19bis</sub>/{coef<sub>envZ</sub>}<sup>n<sub>19bis</sub></sup>) |
| - | |activation constant of | + | |activation constant of EnvZ-pFlhDC <br> replace K<sub>19</sub> (not written) |
| - | |nM<sup>n<sub> | + | |nM<sup>n<sub>19bis</sub></sup> |
| | | | ||
|- | |- | ||
| - | |n<sub> | + | |n<sub>19bis</sub> |
| - | |complexation order of OmpR-pFlhDC <br> n<sub> | + | |complexation order of OmpR-pFlhDC <br> n<sub>19bis</sub> |
|no dimension | |no dimension | ||
| | | | ||
| Line 99: | Line 99: | ||
Then, if we have time, we want to verify the expected relation | Then, if we have time, we want to verify the expected relation | ||
| - | <center>[[Image: | + | <center>[[Image:SumpFlhDC2.jpg]]</center> |
Revision as of 15:46, 21 August 2008
and
so the expression
gives
and
and for calculated values of the TF,
and
| param | signification | unit | value |
| [expr(pFlhDC)] | expression rate of pFlhDC with RBS E0032 | nM.s-1 | see "findparam" need for 20 + 20 measures and 5x5 measures for the SUM? |
| γGFP | dilution-degradation rate of GFP(mut3b) | s-1 | ln(2)/3600 |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the SUM? |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the SUM? |
| conversion | conversion ration between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value |
| β12bis | production rate of FliA-pFlhDC with RBS E0032 replace β12 (not written) | nM.s-1 | |
| (K11bis/{coeffliA}n11bis) | activation constant of FliA-pFlhDC K11bis | nMn11bis | |
| n11bis | complexation order of FliA-pFlhDC replace n11 (not written) | no dimension | |
| β2bis | production rate of EnvZ-pFlhDC with RBS E0032 β2bis | nM.s-1 | |
| (K19bis/{coefenvZ}n19bis) | activation constant of EnvZ-pFlhDC replace K19 (not written) | nMn19bis | |
| n19bis | complexation order of OmpR-pFlhDC n19bis | no dimension |
Then, if we have time, we want to verify the expected relation
