"
Team:Hawaii/Notebook/2008-08-22
From 2008.igem.org
(Difference between revisions)
(→Verification of transformants) |
(margarets stuff) |
||
| Line 43: | Line 43: | ||
:<strong>Grace</strong> | :<strong>Grace</strong> | ||
| - | == | + | ===Ligation=== |
| + | <strong>Margaret</strong> | ||
| + | :*ligation of rep+pSB1A3, P1lytic region+pSB1A3, aada(BB&pRL1383a)+pSB1A3 | ||
| - | === | + | ===omega interposon=== |
| - | : | + | *:streak on Sm&Sp plate to confirm insertion. |
| - | |||
| - | |||
Revision as of 02:40, 23 August 2008
| Projects | Events | Resources | ||
|---|---|---|---|---|
| Sponsors | Experiments | Milestones | Protocols | |
| Notebook (t) | Meetings (t) |
Things we did today
Wetlab work
Verification of transformants
- Grace
| Construct | Colony forming units |
|---|---|
| nir+rbs | 187 |
| plac+rbs | 0 |
| rbs+GFP | 1 |
| GFP+tt | 0 |
- Colony PCR of transformants
- Ran PCR reaction on 2% agarose gel
- Can't tell 15bp difference between GFP and rbs+GFP
- Restreaked single colony and inoculated for plasmid prep to confirm
- nir+rbs colonies #11-19 appear promising (larger than nir)
- Restreaked colony #19 and inoculated for plasmid prep to confirm
Plasmid prep of J33207
- Grace
Overnight RE digest
- Grace
- BB-pRL1383a and J33207 with XbaI and PstI
- pSB1A2 with EcoRI and PstI
- GFP, GFPf, nir with EcoRI and SpeI
Made LB+amp100 plates
- Grace
Ligation
Margaret
- ligation of rep+pSB1A3, P1lytic region+pSB1A3, aada(BB&pRL1383a)+pSB1A3
omega interposon
- streak on Sm&Sp plate to confirm insertion.
Discussion
Quote of the Day
History is the only laboratory we have in which to test the consequences of thought. - Étienne Gilson
[http://manoa.hawaii.edu/ 
][http://manoa.hawaii.edu/ovcrge/ 
][http://www.ctahr.hawaii.edu 
]
