Edinburgh/7 July 2008

From 2008.igem.org

(Difference between revisions)
 
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<html>
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<div id="header">{{Template:Team:Edinburgh/Templates/notebook-entry/header}}</div>
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<head>
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<title>Edinburgh iGEM 2008</title>
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<script type="text/javascript">
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
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//Created: May 16th, 07'
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<style>
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.ddcolortabs{
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width: 100%;
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background: transparent;
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voice-family: "\"}\"";
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.ddcolortabs a{
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color: white;
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background: black url(media/color_tabs_left.gif) no-repeat left top;
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margin:0 2px 0 0;
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text-decoration:none;
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.ddcolortabs a span{
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.ddcolortabs a:hover{
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.ddcolortabs a:hover span{
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background-color: #591f20;
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
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background-color: #591f20;
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.ddcolortabsline{
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clear: both;
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padding: 0;
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width: 100%;
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height: 8px;
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line-height: 8px;
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background: black;
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
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}
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/* ######### Style for Drop Down Menu ######### */
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.dropmenudiv_a{
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position:absolute;
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top: 0;
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border: 1px solid black; /*THEME CHANGE HERE*/
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border-top-width: 8px; /*Top border width. Should match height of .ddcolortabsline above*/
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border-bottom-width: 0;
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font:normal 12px Arial;
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line-height:18px;
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z-index:100;
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background-color: white;
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width: 200px;
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.dropmenudiv_a a{
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width: auto;
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display: block;
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border-top: 0 solid #678b3f;
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border-bottom: 1px solid #678b3f; /*THEME CHANGE HERE*/
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text-decoration: none;
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color: black;
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* html .dropmenudiv_a a{ /*IE only hack*/
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width: 100%;
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
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background-color: #8a3c3d;
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</style>
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</head>
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<!-- CSS for Drop Down Tabs Menu #1 -->
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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</ul>
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</div>
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<div class="ddcolortabsline">&nbsp;</div>
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<div id="dropmenu1_a" class="dropmenudiv_a">
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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tabdropdown.init("colortab", 3)
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<table>
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<html>
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<style type="text/css">
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table.calendar          { margin: 0; padding: 2px; }
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table.calendar td      { margin: 0; padding: 1px; vertical-align: top; }
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table.month .heading td { padding:1px; background-color: black; color: white; text-align:center; font-size:140%; font-weight:bold; }
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table.month .dow td    { color:#000000; text-align:center; font-size:100%; }
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table.month td.today    { background-color:#cd0000; }
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#bodyContent table.month a { background:none; padding:0 }
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</style>
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</html>
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{|style="font color="#ffffff"; "background-color:"#cd0000"; cellpadding="0" cellspacing="4" border="4" bordercolor="#000"; border-spacing:0px; text-align:"center" width="250px"
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</table>
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{| align="left"
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=06}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=07}}
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|-
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|align="left" width="150pt"|{{#calendar: title=Edinburgh |year=2008 | month=08}}
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|}
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:::: '''[[Edinburgh/6_July_2008|< Previous Entry]]'''
:::: '''[[Edinburgh/6_July_2008|< Previous Entry]]'''

Latest revision as of 14:06, 28 August 2008

< Previous Entry

Week 4

Monday 7 July 08

  • Several more white colonies have grown on plate 19 (pSB1A2+appY), but quite small and close together. Tried to sub these to Plate 21.
  • Nimisha and the team did two maxipreps - X1 (J33201), and X2 (the dxs clone of miniprep M2). X2 digested with HindIII (single restriction digest).
  • Purified the mutagenic PCR reactions P6 (BABEL2+glgC-mut1) and P7 (BABEL2+glgC-mut2), and set up self ligations (L6 and L7).
  • Sequence results for glgC clones M10 and M11 show that both are in reverse orientation. The result for the dxs clones M2 looks OK at first glance, but can't see restriction sites since sequencing primers pSB1A2insf1 and pSB1A2insr1 were used rather than pSB1A2f2 and pSB1A2r2.


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