Edinburgh/5 August 2008
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* M72 (pSB1A2+rbs+''dxs''), M79 (BABEL2+''crtE'') and M82 (pSB1A2+rbs+''appY'') submitted for seqencing. (CF) | * M72 (pSB1A2+rbs+''dxs''), M79 (BABEL2+''crtE'') and M82 (pSB1A2+rbs+''appY'') submitted for seqencing. (CF) | ||
* Minipreps '''M97-M104''' of pSB1A2+''cenA'' (plate 85, steaks 2 and 7-13) and '''M105-M108''' of pSB1A2+''cex'' (plate 86) were made. (Yan) | * Minipreps '''M97-M104''' of pSB1A2+''cenA'' (plate 85, steaks 2 and 7-13) and '''M105-M108''' of pSB1A2+''cex'' (plate 86) were made. (Yan) | ||
- | * M97-M108 were double-digested with EcoRI/PstI and run on '''gel 37'''. The results do not look good except for M97 | + | * M97-M108 were double-digested with EcoRI/PstI and run on '''gel 37'''. The results do not look good except for M97. (Yan) |
* Purification and self-ligation of P50 (3rd mutagenesis of ''glgc''-mut1,2) ('''L30'''). (HX) | * Purification and self-ligation of P50 (3rd mutagenesis of ''glgc''-mut1,2) ('''L30'''). (HX) | ||
* M68-M69 (pSB1A2+''glgc''-mut1,2) and M92 (pSB1A2+''cex'') were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG) | * M68-M69 (pSB1A2+''glgc''-mut1,2) and M92 (pSB1A2+''cex'') were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG) |
Revision as of 11:18, 28 August 2008
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Week 8
Tuesday 5 August 08
- M72 (pSB1A2+rbs+dxs), M79 (BABEL2+crtE) and M82 (pSB1A2+rbs+appY) submitted for seqencing. (CF)
- Minipreps M97-M104 of pSB1A2+cenA (plate 85, steaks 2 and 7-13) and M105-M108 of pSB1A2+cex (plate 86) were made. (Yan)
- M97-M108 were double-digested with EcoRI/PstI and run on gel 37. The results do not look good except for M97. (Yan)
- Purification and self-ligation of P50 (3rd mutagenesis of glgc-mut1,2) (L30). (HX)
- M68-M69 (pSB1A2+glgc-mut1,2) and M92 (pSB1A2+cex) were digested with a) PstI and b) EcoRI/PstI, but gel leaked. Entire process needs repeating tomorrow! (CF, OG)
- Digested P36 (glgC-mut1,2) with Edinbrick1 using Spe1/EcoR1 and ligated (L31). (OG)