Edinburgh/Notebook/PCR products

From 2008.igem.org

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<html>
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<div id="header">{{Template:Team:Edinburgh/Templates/Header}}</div>
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<title>Edinburgh iGEM 2008</title>
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<script type="text/javascript">
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//Drop Down Tabs Menu- Author: Dynamic Drive (http://www.dynamicdrive.com)
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//Created: May 16th, 07'
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//No need to edit beyond here////////////////////////
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dropmenuobj: null, ie: document.all, firefox: document.getElementById&&!document.all, previousmenuitem:null,
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currentpageurl: window.location.href.replace("http://"+window.location.hostname, "").replace(/^\//, ""), //get current page url (minus hostname, ie: http://www.dynamicdrive.com/)
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this.shimobject.style.display=(this.dropmenuobj.style.visibility=="visible")? "block" : "none"
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if (this.enableiframeshim && typeof this.shimobject!="undefined")
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var menuurl=menuurl.replace("http://"+menuurl.hostname, "").replace(/^\//, "")
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init:function(menuid, dselected){
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menuitems[i].onmouseover=function(e){
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var event=typeof e!="undefined"? e : window.event
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tabdropdown.dropit(this, event, this.getAttribute("rel"))
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}
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</script>
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<style>
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.ddcolortabs{
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padding: 0;
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width: 100%;
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background: transparent;
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voice-family: "\"}\"";
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.ddcolortabs a{
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color: white;
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background: black url(media/color_tabs_left.gif) no-repeat left top;
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margin:0 2px 0 0;
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padding:0 0 1px 3px;
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text-decoration:none;
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letter-spacing: 1px;
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float:left;
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display:block;
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background: transparent url(media/color_tabs_right.gif) no-repeat right top;
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padding: 4px 8px 2px 7px;
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.ddcolortabs a span{
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.ddcolortabs a:hover{
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.ddcolortabs a:hover span{
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background-color: #591f20;
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.ddcolortabs .selected a, #ddcolortabs .selected a span{ /*currently selected tab*/
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background-color: #591f20;
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.ddcolortabsline{
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padding: 0;
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width: 100%;
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height: 8px;
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line-height: 8px;
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background: black;
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border-top: 1px solid #fff; /*Remove this to remove border between bar and tabs*/
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}
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/* ######### Style for Drop Down Menu ######### */
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.dropmenudiv_a{
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position:absolute;
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top: 0;
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border: 1px solid black; /*THEME CHANGE HERE*/
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font:normal 12px Arial;
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line-height:18px;
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z-index:100;
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background-color: white;
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.dropmenudiv_a a{
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width: auto;
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text-decoration: none;
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color: black;
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* html .dropmenudiv_a a{ /*IE only hack*/
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width: 100%;
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.dropmenudiv_a a:hover{ /*THEME CHANGE HERE*/
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background-color: #8a3c3d;
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color: white;
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}
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</style>
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</head>
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<!-- CSS for Drop Down Tabs Menu #1 -->
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<link rel="stylesheet" type="text/css" href="ddcolortabs.css" />
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<div id="colortab" class="ddcolortabs">
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<ul>
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<li><a href="https://2008.igem.org/Team:Edinburgh" title="Home"><span>Home</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Project" title="Project" rel="dropmenu1_a"><span>The Project</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Modeling" title="Modelling" rel="dropmenu2_a"><span>Modelling</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Notebook" title="Notebook"><span>Notebook</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Results" title="Results" rel="dropmenu1_a"><span>Results</span></a></li>
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<li><a href="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2008&group=Edinburgh" title="Parts" rel="dropmenu1_a"><span>BioBrick Parts</span></a></li>
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<li><a href="https://2008.igem.org/Team:Edinburgh/Team" title="Team" ><span>The Team</span></a></li>
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</ul>
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</div>
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<div class="ddcolortabsline">&nbsp;</div>
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<div id="dropmenu1_a" class="dropmenudiv_a">
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Overview</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step1</a>
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<a href="https://2008.igem.org/Team:Edinburgh/Team">Step2</a>
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//SYNTAX: tabdropdown.init("menu_id", [integer OR "auto"])
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tabdropdown.init("colortab", 3)
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</html>
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'''P1:''' ''dxs'' from ''Escherichia coli'' JM109. (25.06.08)<br />
'''P1:''' ''dxs'' from ''Escherichia coli'' JM109. (25.06.08)<br />
Line 335: Line 46:
'''P62:''' ''cenA'' from ''C. fimi'' genomic DNA. (10.08.08: CF) - Failed<br />
'''P62:''' ''cenA'' from ''C. fimi'' genomic DNA. (10.08.08: CF) - Failed<br />
'''P63:''' ''cex'' from ''C. fimi'' genomic DNA. (10.08.08: CF) - Failed<br />
'''P63:''' ''cex'' from ''C. fimi'' genomic DNA. (10.08.08: CF) - Failed<br />
-
'''P64:''' ''cex'' from ''C. fimi'' genomic DNA, w/ glycerol, annealing 60°C. Success (11.08.08: AM)<br />
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'''P64:''' ''cex'' from ''C. fimi'' genomic DNA, with 10µl 50% glycerol, annealing 60°C. (11.08.08: AM) - Succesful<br />
'''P65:''' ''cenA'' from ''C. fimi'' genomic DNA, w/ glycerol, annealing 56°C/20s. Success (12.08.08: AM)<br />
'''P65:''' ''cenA'' from ''C. fimi'' genomic DNA, w/ glycerol, annealing 56°C/20s. Success (12.08.08: AM)<br />
'''P66:''' ''zm1'' (ISA1) (12.08.08: CF)<br />
'''P66:''' ''zm1'' (ISA1) (12.08.08: CF)<br />

Revision as of 14:51, 28 August 2008

P1: dxs from Escherichia coli JM109. (25.06.08)
P2: appY from E. coli JM109. (25.06.08)
P3: glgC from E. coli JM109. (25.06.08)
P4: Fusion PCR for BABEL1+glgC. (01.07.08)
P5: Fusion PCR for BABEL2+glgC. (01.07.08)
P6: Mutagenic PCR to remove EcoRI site 1 from glgC (M11). (04.07.08)
P7: Mutagenic PCR to remove EcoRI site 2 from glgC (M11). (04.07.08)
P8: crtB (from Pantoea ananatis cells). (08.07.08)
P9: crtI (from Douglas's maxiprep of the mutated crtIB plasmid). (08.07.08)
P10: crtY (from P. ananatis cells). Failed. (08.07.08)
P11: Fusion PCR of rbs+dxs. Failed. (10.07.08)
P12: Repeat fusion PCR of rbs+dxs, using L11 and primers rbs2fclon1+pSB1A2insr1. (13.07.08)
P13-P14: MABEL mutation of site 1 on glgC-mut2 clones M19 and M22. (14.07.08)
P15: Rusion PCR for rbs+appY using L15 as a template. (15.07.08)
P16-P19: First attempts at cenA, cenB, cenC and cex. (17.07.08) - Failed
P20-P23: Second attempts at cenA, cenB, cenC and cex. (18.07.08) - Failed
P24-P25: Repeat PCR for cenA and cex using Pfu. (19.07.08) - Failed
P26: Positive control PCR with primers fd1 and rd1 to amplify 16S rRNA gene rrnB from C. fimi. (22.07.08: AM) - Failed
P27: crtY from P. ananatis cells. (23.07.08: AM, Yan, OG) - Failed
P28: Fusion PCR of rbs+crtB from L18. (23.07.08: AM, Yan, OG)
P29: Fusion PCR of rbs+crtI from L19. (23.07.08: AM, Yan, OG)
P30: Repeat positive control of rrnB from C. fimi, annealing 1 minute. (23.07.08: AM)
P31: pZntA promoter
P32-P35: Repeat PCR of cenA, cenB, cenC and cex, annealing 1 minute. (24.07.08: AM) - Failed
P36: M43 (glgC-mut1,2) with primers glgCf2/glgCr2. (25.07.08: AM)
P37: crtY from P. ananatis. (25.07.08: AM) - Failed
P38-P41: cenA, cenB, cenC and cex from heat-killed cell suspension. (28.07.08: AM)
P42-P45: cenA, cenB, cenC and cex from 'impure' DNA solution. (28.07.08: AM)
P46-P47: cenA and cex from 'impure' DNA solution, annealing 65C. (20.07.08: AM) - Failed
P48: crtY (new primer mixture) (30.07.08: CF) - Failed
P49: Recreation of P12 (rbs+dxs) (30.07.08: CF)
P50: Third mutagenesis of glgC-mut1,2 by MABEL (04.08.08: Yan)
P51: pCstA from E. coli cells. (06.08.08: CF) - Failed
P52: cenA from heat killed C. fimi. (07.08.08: Yan, AM) - Failed
P53: cex from heat killed C. fimi. (07.08.08: Yan, AM) - Failed
P54: pCstA from E. coli cells (07.08.08: CF)
P55: cenA performed by KOD. (08.08.08: Yan)
P56: cenA performed by velocity. (08.08.08: Yan)
P57: cex performed by KOD. (08.08.08: Yan)
P58: cex performed by Velocity. (08.08.08: Yan)
P59: pSB1A2+crtY (09.08.08: CF)
P60: pSB1A2+rbs+crtY (09.08.08: CF)
P61: 16s rRNA rrnB from C. fimi genomic DNA. (10.08.08: CF) - successful
P62: cenA from C. fimi genomic DNA. (10.08.08: CF) - Failed
P63: cex from C. fimi genomic DNA. (10.08.08: CF) - Failed
P64: cex from C. fimi genomic DNA, with 10µl 50% glycerol, annealing 60°C. (11.08.08: AM) - Succesful
P65: cenA from C. fimi genomic DNA, w/ glycerol, annealing 56°C/20s. Success (12.08.08: AM)
P66: zm1 (ISA1) (12.08.08: CF)
P67: zm2 (ISA2) (12.08.08: CF)
P68: cenB from C. fimi genomic DNA. Failed (13.08.08: AM)
P69: cenC from C. fimi genomic DNA. Failed (13.08.08: AM)
P70: M43 (glgC mut 1+2). Failed (14.08.08: AM)
P71: M120 (glgC mut 1+2+3). Successful (14.08.08: AM)
P71A: Repeat M43 (glgc mut 1+2). Succeessful (15.08.08: AM)
P72: Repeat M120 (glgC mut 1+2+3). Successful (15.08.08: AM)
P73~P77: rrnB from C. fimi genomic DNA purified on 14.08.08. P74 possibly successful (15.08.08: AM)
P78A: rrnB from original C. fimi genomic DNA as positive control. Failed (15.08.08: AM)
P78: SOB2-glgC mut 1+2 from L45 (19.08.08: AM)
P79: SOB2-glgC mut 1+2+3 from L46 (19.08.08: AM)
P80: SOB2-glgC mut 1+2+3 from L47 (19.08.08: AM)
P81: SOB2-rbs-glgc mut 1+2 from L45 (19.08.08: AM)
P82: SOB2-rbs-glgC mut 1+2+3 from L46 (19.08.08: AM)
P83: SOB2-rbs-glgC mut 1+2+3 from L47 (19.08.08: AM)
P84: zm1 (ISA1) from X11 (20.08.08: AM)
P85: zm2 (ISA2) from X12 (20.08.08: AM)

Retrieved from "http://2008.igem.org/Edinburgh/Notebook/PCR_products"