Edinburgh/Notebook/Plates

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Revision as of 21:28, 6 October 2008

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Overview Step1 Step2

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Plates

Plates 1-2: transformation with a BioBrick from the Registry stock: failed. (18.06.08)
Plate 3: L1 (pSB1A2+dxs) transformation, 100μl. (27.06.08)
Plate 4: L2 (pSB1A2+appY) transformation, 100μl. (27.06.08) - Failed
Plate 5: L3 (BABEL1+glgC) transformation, 100μl. (27.06.08)
Plate 6: L4 (BABEL2+glgC) transformation, 100μl. (27.06.08)
Plate 7: L1 (pSB1A2+dxs) transformation, 900μl. (27.06.08)
Plate 8: L2 (pSB1A2+appY) transformation, 900μl. (27.06.08) - Failed
Plate 9: L3 (BABEL1+glgC) transformation, 900μl. (27.06.08)
Plate 10: L4 (BABEL2+glgC) transformation, 900μl. (27.06.08)
Plate 11: possible pSB1A2+dxs transformants. (28.06.08)
Plate 12: possible Babel1+glgC transformants. (28.06.08)
Plate 13: possible Babel2+glgC transformants. (28.06.08)
Plate 14: more subcultures from plates 5 and 6. (29.06.08)
Plates 15-16: retransformation of religated L2. Failed again. (03.07.08)
Plate 17: Pantoea ananatis for crt PCR reactions. (05.07.08)
Plates 18-19: L5 (pSB1A2+appY) transformation, 100μl and 900μl. (05.07.08)
Plate 20: subculture from plate 19. (06.07.08)
Plate 21: subcultures from plate 19. (07.07.08)
Plates 22-23: L6 (BABEL2+glgC-mut1) transformation. No colonies. (08.07.08)
Plates 24-25: L7 (BABEL2+glgC-mut2) transformation. (08.07.08)
Plates 26-27: subcultures from plates 24 and 25 (L7). (09.07.08)
Plates 28-29: Cellulomonas fimi from initial stock. (11.07.08)
Plates 30-31: possible pSB1A2+crtB (L9) transformants. (11.07.08)
Plates 32-33: possible pSB1A2+crtI-mut (L10) transformants. (11.07.08)
Plate 34: possible pSB1A2+rbs+dxs clones from Nimisha's plates. (11.07.08)
Plate 35: patches of possible pSB1A2+crtB transformants from Plate 30. (12.07.08)
Plate 36: patches of possible pSB1A2+crtI-mut transformants from Plates 32 and 33. (12.07.08)
Plate 37: subculture of C. fimi on NA with filter paper (cellulose). (15.07.08)
Plates 38-39: L15 transformation (pSB1A2+rbs+dxs). (15.07.08)
Plates 40-41: L13 transformation (BABEL2+glgC-mut1,2). (15.07.08)
Plates 42, 44 and 45: Patches of possible BABEL2+glgC-mut1,2 transformants - subcultures from plate 41. (17.07.08)
Plate 43: Patches of possible pSB1A2+rbs+dxs transformants - subcultures from plate 38 or 39. (17.07.08)
Plates 46-47: L16 transformation (rbs+appY with Edinbrick1) (18.07.08) - No growth
Plates 48-50: Cellulomonas fimi from overnight cultures made from plate 37. (24.07.08: AM)
Plates 51-52: L19 (pSB1A2+rbs+dxs) transformation. (26.07.08: CF) - No growth
Plates 53-54: L20 (pSB1A2+rbs+appY) transformation. (26.07.08: CF) - No growth
Plates 55-56: L21 (pSB1A2+rbs+crtB) transformation. (26.07.08: CF) - No growth
Plates 57-58: L22 (pSB1A2+rbs+crtI) transformation. (26.07.08: CF)
Plates 59-60: L23 (pSB1A2+pZntA) transformation. (26.07.08: CF)
Plates 61-62: L24 (pSB1A2+crtE - from BABEL2) transformation. (26.07.08: CF)
Plates 63-64: Repeat transformation of L20 (pSB1A2+rbs+appY). (28.07.08: Yan, OG) - No growth
Plates 65-66: Repeat transformation of L21 (pSB1A2+rbs+crtB). (28.07.08: Yan, OG)
Plates 67-68: L25 (pSB1A2+glgC-mut1,2) transformation. (29.07.2008: Yan)
Plates 69-70: pSB1A2+rbs+dxs ligation. (30.07.2008: CF)
Plates 71-72: ligation of crtE to BABEL2. (30.07.2008: CF)
Plate 73: Subs from plate 67 (pSB1A2+glgC-mut1,2) (30.07.2008: CF)
Plate 74: Subs from plates 69-72 (pSB1A2+rbs+dxs and BABEL2+crtE) (30.07.2008: CF)
Plates 75-76: L26 (pSB1A2+rbs+appY) transformation. (31.07.2008: HX)
Plates 77-78: L27 (pSB1A2+rbs+crtB) transformation. (31.07.2008: HX)
Plates 79-80: L28 (pSB1A2+cenA) transformation' (31.07.2008: HX)
Plates 81-82: L29 (pSB1A2+cex) transformation' (31.07.2008: HX)
Plate 83: Subs from Plate 76 (L26: pSB1A2+rbs+appY) (01.08.2008: OG)
Plate 84: Subs from Plate 77 (L27: pSB1A2+rbs+crtB) (01.08.2008: HX)
Plate 85: Subs from Plate 80 (L28: pSB1A2+cenA) (01.08.2008: AM)
Plate 86: Subs from Plates 81-82 (L29: pSB1A2+cex) (01.08.2008: AM)
Plates 89-90: L30 (Self-ligation of BABEL2+glgC-mut1,2,3) transformation. (06.08.2008: HX)
Plates 91-92: L31 (glgC-mut1,2 to Edinbrick1) transformation. (06.08.2008: HX)
Plate 93: Subculture from Plate 56 (M67, pSB1A2+rbs+crtB). (06.08.08: CF)
Plate 94: Subcultures from Plates 89-90 (BABEL2+glgC-mut1,2,3). (07.08.08: HX)
Plate 95: Subculture from Plate 92 (pSB1A2+glgC-mut1,2). (07.08.08: HX)
Plate 96: LB, ampicillin plate spread with subcultures from plate 43 (pSB1A2+rbs+dxs). (07.08.08: HX)
Plate 97: LB, ampicillin, 2% glucose plate spread with subcultures from plate 43 (pSB1A2+rbs+dxs). (07.08.08: HX)
Plates 98-99: Transformation of L32 (pSB1A2+crtB+crtI). (08.08.08: HX)
Plates 100-101: Repeat transformation of L31 (glgC-mut1,2 to Edinbrick1) (08.08.08: HX)
Plate 102: Patches from plate 99 (L32, pSB1A2+crtB+crtI). (09.08.08: CF)
Plate 103: Patches from plate 101 (L31, pSB1A2+glgC-mut1,2). (09.08.08: CF)
Plate 104-105: Transformation of L36 (pSB1A2+crtY) 100µl/900µl. (10.08.08: CF)
Plate 106-107: Transformation of L37 (pSB1A2+rbs+crtY) 100µl/900µl. (10.08.08: CF)
Plate 108: Subcultures from plates 105 and 106. (11.08.08: CF)
Plates 109-110: Transformation of L33 (pBS1A2+pCstA) 100µl/900µl (11.08.08: Yan)
Plates 111-112: Transformation of L34 (pBS1A2+dxs+crtE) 100µl/900µl (11.08.08: Yan)
Plates 113-114: Transformation of L35 (pBS1A2+dxs+lims1) 100µl/900µl (11.08.08: Yan)
Plate 115: Subs from L33 (Pl. 109/110: pSB1A2-PcstA), L34 (Pl. 111/112: dxs-crtE) and L35 (Pl. 113/114: dxs-lims)
Plates 116/117: Transformation of L38 (psb1A2\-_crtE_) (13.08.08: HX)
Plate 118: Subs from plate 116 (pSB1A2+crtE 100 microlitres). (14.08.08: AH)
Plate 119/120: Transformation of L40 (CenA+Edinbrick1) (14.08.08: YAN)
Plate 121/122: Transformation of L39 (Cex+Edinbrick1) (14.08.08: YAN)
Plate 123/124: Repeat to transform with L39 (cex+Edinbrick1)  (15.08.08: HX)
Plate 125/126: Transform with L40 (cenA+Edinbrick1) (15.08.08: HX)
Plate 127/128: Transform with L41 (dxs/crtE) (15.08.08: HX)
Plate 129: CenA & CeX white colonies from Plates 120 & 123-126 (16/08: CF)
Plate 130: dxs-CrtE white colonies from plate 127-128 (16/08: CF)
Plate 131/132: maize pENTR221 transformation, ISA1 Kan 10 (16/08: CF)
Plate 133/134: maize pENTR221 transformation, ISA2 Kan 10 (16/08: CF)
Plate 135: patches(colonies) from plates 131,133 (16/08: CF)
Plate 136/137: Transformation with L48 (dxs+Lims+Appy) (20.08.08: Yan)
Plate 138/139: Transformation with L49 (CrtBI+Appy) (20.08.08: YAN)
Plate 140: Transformation with Edinbrick1 only as control (20.08.08: YAN)
Plates 141~147: Transformations of L50~L56, 100ml (21.08.08: AM)
Plates 148~154: Transformations of L50~L56, 900ml (21.08.08: AM)
Plate 155: Subs from plates 137-139. (21.08.08: AH)
Plate 158: Subs from plates 141, 148: L50 (SOB2-glgC mut 1+2) (22.08.08: AM)
Plate 159: Subs from plates 142, 149: L51 (SOB2-glgC mut 1+2+3) (22.08.08: AM)
Plate 160: Subs from plates 143, 150: L52 (SOB2-glgC mut 1+2+3); subs from plate 153: L55 (SOB2-rbs-glgC mut 1+2+3 (22.08.08: AM)
Plate 161: Subs from plates 144, 151: L53 (SOB2-rbs-glgC mut 1+2) (22.08.08: AM)
Plate 162: Subs from plates 147, 154: L56 (LacZ-PcstA) WHITE colonies (22.08.08: AM)
Plate 163: Subs from plates 154: L56 (LacZ-PcstA) BLUE colonies (22.08.08: AM)
Plate 164: 18 subs from plate 126 (pSB1A2+cenA) (28.08.08: AH)
Plate 165: pSB1A2-CHU2268 (beta glucosidase, from P90), 100µl (28.08.08: AM)
Plate 166: pSB1A2-CHU2268 (beta glucosidase, from P90), 900µl (28.08.08: AM)
Plate 167: Transformation of L57 (AppY into CrtBI),100µl (29.08.08: YAN)
Plate 168: Transformation of L57 (AppY into CrtBI),900µl (29.08.08: YAN)
Plate 169: Sub-clone from Plate 167-168 (30.08.09: CF)
Plates 170/171: Transformation of L58 (04.09.08: CF)
Plates 172/173: Transformation of L59 (04.09.08: CF)
Plates 174/175: Transformation of L60 (04.09.08: CF)
Plates 176/177: Transformation of L61 (04.09.08: CF)
Plate 178: sub-clone from L58, L59 (05.09.08: CF)
Plate 179: sub-clone from L60, L61 (05.09.08: CF)
Plates 180/181: Transformation of L62 (dxs+crtE into CerBI) (10.09.08: YAN)
Plates 182/183: Transformation of L63 (P90 into Edin1) (10.09.08: YAN)
Plates 184/185: Transformation of L64 (CenA into Edin1) (10.09.08: YAN)
Plates 188/189: Repeat transformation with L62 (dxs+crtE into CrtBI), 100/900µl (11.09.08: AM)
Plates 190/191: Repeat transformation with L63 (pSB1A2-CHU2268), 100/900µl (11.09.08: AM)
Plates 192/193: Repeat transformation with L64 (pSB1A2-cenA), 100/900µl (11.09.08: AM)
Plate 194: Transformation with Edinbrick 1 as control, 900µl (11.09.08: AM)
Plate 195: Possible SOB-glgC triple mutant subs from plates 186-7 (12.09.08: CF)
Plate 196: Possible dxs+crtEB2 subs from plate 181 (12.09.08: CF)
Plate 197: Possible cenA+bglX subs from plates 183, 185 (12.09.08: CF)
Plate 198: Possible dxs+CrtE into CrtBI subs from plates 188, 189 (12.09.08: AM)
Plate 199: Possible pSB1A2-CHU2268 subs from plate 191 (12.08.08: AM)
Plate 200/201: Transformation of L65 (Lac promotor+dxs+Lims+Appy) (17.09.08:CF)
Plate 202/203: Transformation of L66 (PcstA+LacZ') (17.09.08:CF)
Plate 204: control.

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