Team:Chiba/protocol/gelcheck

From 2008.igem.org

(Difference between revisions)
 
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>[[Team:Chiba/protocol|Protocol]]
>[[Team:Chiba/protocol|Protocol]]
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Gel Check
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Agarose gel electrophoresis
 +
 
 +
*Agalose Gel casting
 +
#Measure out the appropriate mass of agarose into glass bottle with the appropriate volume of TAE buffer
 +
#Microwave until the agarose is fully melted
 +
#Pour the agarose solution into the gelbox and let it cool for about 30 minutes, until the gel is solid
 +
#Remove comb
 +
 
 +
 
 +
*Running agalose gel
 +
 
 +
#Load 5 μL prepared 1kbp ladder
 +
#Mix DNA solution with loading dye(6x) and water
 +
#Load it into agalose gel
 +
#Run the gel at ~100 volts for 35 mins.
 +
 
 +
*Visualizing agarose gels
 +
#Remove gel from gel box
 +
#Soak the gel in ethidium bromide solution
 +
#Let it 30 min.
 +
#Place the gel in Trans-Illuminator and turn on UV light after make sure the door closing.
 +
#Print the picture.
 +
#Remove gel and throw in trash
 +
#Wipe down Trans-Illuminator if necessary.

Latest revision as of 02:43, 11 September 2008

>Protocol

Agarose gel electrophoresis

  • Agalose Gel casting
  1. Measure out the appropriate mass of agarose into glass bottle with the appropriate volume of TAE buffer
  2. Microwave until the agarose is fully melted
  3. Pour the agarose solution into the gelbox and let it cool for about 30 minutes, until the gel is solid
  4. Remove comb


  • Running agalose gel
  1. Load 5 μL prepared 1kbp ladder
  2. Mix DNA solution with loading dye(6x) and water
  3. Load it into agalose gel
  4. Run the gel at ~100 volts for 35 mins.
  • Visualizing agarose gels
  1. Remove gel from gel box
  2. Soak the gel in ethidium bromide solution
  3. Let it 30 min.
  4. Place the gel in Trans-Illuminator and turn on UV light after make sure the door closing.
  5. Print the picture.
  6. Remove gel and throw in trash
  7. Wipe down Trans-Illuminator if necessary.
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